The Effect of the Oxygen Scavenging System on the pH of Buffered Sample Solutions: in the Context of Single-molecule Fluorescence Measurements
DC Field | Value | Language |
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dc.contributor.author | Kim, Sung Eun | - |
dc.contributor.author | Lee, Il Buem | - |
dc.contributor.author | Hong, Seok-Cheol | - |
dc.date.accessioned | 2021-09-06T08:10:34Z | - |
dc.date.available | 2021-09-06T08:10:34Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2012-03-20 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/105286 | - |
dc.description.abstract | In single-molecule fluorescence experiment, the oxygen scavenging system is indispensable for avoiding photo-bleaching of fluorescent dyes. Here we report that the gloxy-based oxygen scavenging system commonly used in single molecule fluorescence experiments can disturb the solution pH considerably. To track in situ pH change, we utilized the pH-sensitive conformational transition of i-motif and examined the transition with ensemble and single-molecule FRET measurements. Based on our results, we also suggested several practical remedies for the stability of the solution pH. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN CHEMICAL SOC | - |
dc.title | The Effect of the Oxygen Scavenging System on the pH of Buffered Sample Solutions: in the Context of Single-molecule Fluorescence Measurements | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Seok-Cheol | - |
dc.identifier.doi | 10.5012/bkcs.2012.33.3.958 | - |
dc.identifier.scopusid | 2-s2.0-84863345111 | - |
dc.identifier.wosid | 000302196300033 | - |
dc.identifier.bibliographicCitation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.33, no.3, pp.958 - 962 | - |
dc.relation.isPartOf | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.title | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.volume | 33 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 958 | - |
dc.citation.endPage | 962 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001653027 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordAuthor | Oxygen scavenging system | - |
dc.subject.keywordAuthor | pH | - |
dc.subject.keywordAuthor | Single-molecule fluorescence | - |
dc.subject.keywordAuthor | i-motif | - |
dc.subject.keywordAuthor | Gloxy | - |
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