Highly Efficient Enzyme Immobilization and Stabilization within Meso-Structured Onion-Like Silica for Biodiesel Production
- Authors
- Jun, Seung-Hyun; Lee, Jinwoo; Kim, Byoung Chan; Lee, Ji Eun; Joo, Jin; Park, Hyunmin; Lee, Jong Ho; Lee, Sang-Mok; Lee, Dohoon; Kim, Sangyong; Koo, Yoon-Mo; Shin, Chae Ho; Kim, Seung Wook; Hyeon, Taeghwan; Kim, Jungbae
- Issue Date
- 13-3월-2012
- Publisher
- AMER CHEMICAL SOC
- Keywords
- meso-structured onion-like silica; enzyme immobilization; enzyme stabilization; biodiesel production
- Citation
- CHEMISTRY OF MATERIALS, v.24, no.5, pp.924 - 929
- Indexed
- SCIE
SCOPUS
- Journal Title
- CHEMISTRY OF MATERIALS
- Volume
- 24
- Number
- 5
- Start Page
- 924
- End Page
- 929
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/105299
- DOI
- 10.1021/cm202125q
- ISSN
- 0897-4756
- Abstract
- Meso-structured onion-like silica (Meso-Onion-S) was synthesized and used as a host of enzyme immobilization. Meso-Onion-S has a 200-300 nm sized primary meso-structured onion building unit, and each onion unit has highly curved mesopores of 10 nm diameter in a multishell structure. Nanoscale enzyme reactors (NERs) in Meso-Onion-S were prepared via a two-step process of enzyme adsorption and subsequent enzyme cross-linking, which effectively prevents the leaching of cross-linked enzyme aggregates from highly curved mesopores of Meso-Onion-S. As a result, NERs in Meso-Onion-S significantly improved the enzyme stability as well as the enzyme loading. For example, NER of lipase (NER-LP) was stable under rigorous shaking for 40 days, while the control sample of adsorbed LP (ADS-LP) with no enzyme cross-linking showed a rapid inactivation due to rigorous enzyme leaching under shaking. Stable NER-LP was successfully employed to produce biodiesels and fatty acid methyl esters, from the LP-catalyzed transesterification of soybean oil with methanol. Interestingly, the specific activity of NER-LP was 23 and 10 times higher than those of free LP and ADS-LP, respectively, revealing the importance of LP stabilization in the form of NER-LP in the presence of organic solvents.
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