Transcriptional analysis of infiltrating T cells in kidney ischemia-reperfusion injury reveals a pathophysiological role for CCR5
DC Field | Value | Language |
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dc.contributor.author | Ko, Gang Jee | - |
dc.contributor.author | Linfert, Douglas | - |
dc.contributor.author | Jang, Hye Ryoun | - |
dc.contributor.author | Higbee, Elizabeth | - |
dc.contributor.author | Watkins, Tonya | - |
dc.contributor.author | Cheadle, Chris | - |
dc.contributor.author | Liu, Manchang | - |
dc.contributor.author | Racusen, Lorraine | - |
dc.contributor.author | Grigoryev, Dmitry N. | - |
dc.contributor.author | Rabb, Hamid | - |
dc.date.accessioned | 2021-09-06T08:20:47Z | - |
dc.date.available | 2021-09-06T08:20:47Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2012-03 | - |
dc.identifier.issn | 1931-857X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/105346 | - |
dc.description.abstract | Ko GJ, Linfert D, Jang HR, Higbee E, Watkins T, Cheadle C, Liu M, Racusen L, Grigoryev DN, Rabb H. Transcriptional analysis of infiltrating T cells in kidney ischemia-reperfusion injury reveals a pathophysiological role for CCR5. Am J Physiol Renal Physiol 302: F762-F773, 2012. First published December 7, 2011; doi:10.1152/ajprenal.00335.2011.-Although T cells have been shown to play a direct role in kidney ischemia-reperfusion injury (IRI), little is known about the underlying mechanisms. We hypothesized that studying the transcriptional responses in kidney-infiltrating T cells would help elucidate novel therapeutic targets for kidney IRI. Unilateral renal pedicle clamping for 45 min was performed in male C57BL/6 mice, and CD3(+) T cells were isolated from the kidney and purified. Transcriptional activities of T cell were measured by array-based PCR compared between ischemic kidneys and contralateral nonischemic kidneys. Among total of 89 genes analyzed, 24, 22, 24, and 37 genes were significantly changed at 6 h, day 3, day 10, and day 28 after IRI. Genes associated with cytokines, chemokines, and costimulatory molecules were upregulated. Pathway analysis identified CC motif chemokine receptor 5 (CCR5) as a candidate pathophysiological pathway. CCR5 upregulation was validated at the protein level, and CCR5 blockade improved renal function after kidney IRI. Using discovery techniques to identify transcriptional responses in purified kidney-infiltrating cells enabled the elucidation of novel mechanisms and therapeutic targets for IRI. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER PHYSIOLOGICAL SOC | - |
dc.subject | RENAL ISCHEMIA/REPERFUSION INJURY | - |
dc.subject | DELAYED GRAFT FUNCTION | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | DIABETIC-NEPHROPATHY | - |
dc.subject | LUNG TRANSPLANTATION | - |
dc.subject | LIVER ISCHEMIA | - |
dc.subject | RECEPTOR | - |
dc.subject | LYMPHOCYTES | - |
dc.subject | IDENTIFICATION | - |
dc.subject | INFLAMMATION | - |
dc.title | Transcriptional analysis of infiltrating T cells in kidney ischemia-reperfusion injury reveals a pathophysiological role for CCR5 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ko, Gang Jee | - |
dc.identifier.doi | 10.1152/ajprenal.00335.2011 | - |
dc.identifier.scopusid | 2-s2.0-84858275288 | - |
dc.identifier.wosid | 000301790200013 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, v.302, no.6, pp.F762 - F773 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | - |
dc.citation.title | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | - |
dc.citation.volume | 302 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | F762 | - |
dc.citation.endPage | F773 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.subject.keywordPlus | RENAL ISCHEMIA/REPERFUSION INJURY | - |
dc.subject.keywordPlus | DELAYED GRAFT FUNCTION | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | DIABETIC-NEPHROPATHY | - |
dc.subject.keywordPlus | LUNG TRANSPLANTATION | - |
dc.subject.keywordPlus | LIVER ISCHEMIA | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | LYMPHOCYTES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordAuthor | acute kidney injury | - |
dc.subject.keywordAuthor | T lymphocyte | - |
dc.subject.keywordAuthor | array-based QRT-PCR | - |
dc.subject.keywordAuthor | chemokine receptor 5 | - |
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