Effect of Gamma Radiation on Growth and Lignin Content in Brachypodium distachyonEffect of Gamma Radiation on Growth and Lignin Content in Brachypodium distachyon
- Other Titles
- Effect of Gamma Radiation on Growth and Lignin Content in Brachypodium distachyon
- Authors
- 이만보; 서용원; 김대연; 전웅배; 홍민정; 이영진; Odgerel Bold; 장지희; 임윤정; 감시영; 김동섭; 김진백; 천재범
- Issue Date
- 2013
- Publisher
- 한국작물학회
- Keywords
- Brachypodium distachyon; gamma radiation; lignin biosynthesis; mutant
- Citation
- Journal of Crop Science and Biotechnology, v.16, no.2, pp.105 - 110
- Indexed
- KCI
- Journal Title
- Journal of Crop Science and Biotechnology
- Volume
- 16
- Number
- 2
- Start Page
- 105
- End Page
- 110
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/105769
- DOI
- 10.1007/s12892-013-0022-9
- ISSN
- 1975-9479
- Abstract
- Brachypodium distachyon has been highlighted as a model monocot plant with small genome and short life cycle. Biofuels are being developed as renewable energy sources to replace fossil fuels. Bioethanol production is negatively correlated with lignin content.
Here, Brachypodium was acutely or chronically irradiated at doses of 50, 100, 150, 200, and 250 Gy. The effect of radiation on plant growth and generation of mutant populations was explored. The lethal effect of radiation was higher in acutely irradiated M0populations. A dose-dependent negative effect in plant height, tiller number, floral spikelet, and total seed number was observed,with a positive effect in days to heading. The phenotype of 1,773 M1 plants was evaluated, with 417 plants being selected to construct the M2 population. The 31 M2 plants that showed the least staining with phloroglucinol were selected. These mutants could be useful materials for studies such as identification of nucleotide substitutions in genes involved in lignin biosynthesis pathway, monitoring of mutant physiological traits, and evaluation of fitness for bioethanol production. As biological resources, the M2 populations generated in this work will contribute to studies of functional genomics of Brachypodium and to the breeding of grass crops
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Collections - Graduate School > Department of Plant Biotechnology > 1. Journal Articles
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