Three-dimensional differential interference contrast microscopy using synthetic aperture imaging
- Authors
- Kim, Moonseok; Choi, Youngwoon; Fang-Yen, Christopher; Sung, Yongjin; Kim, Kwanhyung; Dasari, Ramachandra R.; Feld, Michael S.; Choi, Wonshik
- Issue Date
- 2월-2012
- Publisher
- SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
- Keywords
- image processing; interferometry; microscopy; synthetic apertures
- Citation
- JOURNAL OF BIOMEDICAL OPTICS, v.17, no.2
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF BIOMEDICAL OPTICS
- Volume
- 17
- Number
- 2
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/106106
- DOI
- 10.1117/1.JBO.17.2.026003
- ISSN
- 1083-3668
- Abstract
- We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.2.026003]
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- Appears in
Collections - College of Science > College of Science > 1. Journal Articles
- College of Science > Department of Physics > 1. Journal Articles
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