Solution structure and stability of the DNA undecamer duplexes containing oxanine mismatch
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pack, Seung Pil | - |
dc.contributor.author | Morimoto, Hirohisa | - |
dc.contributor.author | Makino, Keisuke | - |
dc.contributor.author | Tajima, Kunihiko | - |
dc.contributor.author | Kanaori, Kenji | - |
dc.date.accessioned | 2021-09-06T10:37:59Z | - |
dc.date.available | 2021-09-06T10:37:59Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2012-02 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/106187 | - |
dc.description.abstract | Solution structures of DNA duplexes containing oxanine (Oxa, O) opposite a cytosine (O:C duplex) and opposite a thymine (O:T duplex) have been solved by the combined use of H-1 NMR and restrained molecular dynamics calculation. One mismatch pair was introduced into the center of the 11-mer duplex of [d(GTGACO(6)CACTG)/d(CAGTGX(17)GTCAC), X = C or T]. H-1 NMR chemical shifts and nuclear Overhauser enhancement (NOE) intensities indicate that both the duplexes adopt an overall right-handed B-type conformation. Exchangeable resonances of C-17 4-amino proton of the O:C duplex and of T-17 imino proton of O:T duplex showed unusual chemical shifts, and disappeared with temperature increasing up to 30 degrees C, although the melting temperatures were > 50 degrees C. The O:C mismatch takes a wobble geometry with positive shear parameter where the Oxa ring shifted toward the major groove and the paired C-17 toward the minor groove, while, in the O:T mismatch pair with the negative shear, the Oxa ring slightly shifted toward the minor groove and the paired T-17 toward the major groove. The Oxa mismatch pairs can be wobbled largely because of no hydrogen bond to the O1 position of the Oxa base, and may occupy positions in the strands that optimize the stacking with adjacent bases. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | CENTER-DOT-T | - |
dc.subject | BASE-PAIR MISMATCHES | - |
dc.subject | NITRIC-OXIDE | - |
dc.subject | NITROUS-ACID | - |
dc.subject | NMR | - |
dc.subject | DYNAMICS | - |
dc.subject | REPAIR | - |
dc.subject | PH | - |
dc.subject | THERMODYNAMICS | - |
dc.subject | CONFORMATION | - |
dc.title | Solution structure and stability of the DNA undecamer duplexes containing oxanine mismatch | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1093/nar/gkr872 | - |
dc.identifier.scopusid | 2-s2.0-84857837909 | - |
dc.identifier.wosid | 000301069400043 | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.40, no.4, pp.1841 - 1855 | - |
dc.relation.isPartOf | NUCLEIC ACIDS RESEARCH | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 40 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1841 | - |
dc.citation.endPage | 1855 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | CENTER-DOT-T | - |
dc.subject.keywordPlus | BASE-PAIR MISMATCHES | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | NITROUS-ACID | - |
dc.subject.keywordPlus | NMR | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | PH | - |
dc.subject.keywordPlus | THERMODYNAMICS | - |
dc.subject.keywordPlus | CONFORMATION | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.