Isolation of Side Population Cells in B-Cell Non-Hodgkin's Lymphomas
- Authors
- Lee, Mi Ran; Ju, Hyun-Jeong; Kim, Byung Soo; Ko, Young Hyeh; Kim, Won Seog; Kim, Seok Jin
- Issue Date
- 2013
- Publisher
- KARGER
- Keywords
- B-cell lymphoma; Cancer stem cells; Non-Hodgkin' s lymphoma; Side population; Stem cell-like factors
- Citation
- ACTA HAEMATOLOGICA, v.129, no.1, pp.10 - 17
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACTA HAEMATOLOGICA
- Volume
- 129
- Number
- 1
- Start Page
- 10
- End Page
- 17
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/106559
- DOI
- 10.1159/000341284
- ISSN
- 0001-5792
- Abstract
- Background: Side population (SP) cells are characterized by the ability to exclude Hoechst 33342 dye due to high expression of the ATP-binding cassette transporter. This ability is associated with drug-resistant characteristics of cancer stem cells. Methods: We analyzed SP cells from human B-cell non-Hodgkin's lymphoma cell lines and primary cells derived from patients and compared them with non-SP (NSP) cells. Results: SP cells comprised a minor fraction of all cells ranging from 1.5 +/- 1.8 to 8.3 +/- 5.7% in cell lines and had higher ABCG2 expression than NSP cells. SP cells had better cell viability, colony-forming ability and drug resistance than NSP cells. The SP cells also showed stem cell-like characteristics, including elevated telomerase activity and higher expression of OCT4 and NANOG. A cDNA microarray demonstrated that SP cells had decreased expression of genes associated with apoptosis and cell death compared to NSP cells. Conclusions: The presence of SP cells might imply the possibility of lymphoma stem cells and be associated with a malignant potential of B-cell lymphoma. Copyright (C) 2012 S. Karger AG, Basel
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