Polymeric LabChip Real-Time PCR as a Point-of-Care-Potential Diagnostic Tool for Rapid Detection of Influenza A/H1N1 Virus in Human Clinical Specimens
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Hyun-Ok | - |
dc.contributor.author | Kim, Je-Hyoung | - |
dc.contributor.author | Ryu, Ho-Sun | - |
dc.contributor.author | Lee, Dong-Hoon | - |
dc.contributor.author | Kim, Sun-Jin | - |
dc.contributor.author | Kim, Deog-Joong | - |
dc.contributor.author | Suh, In Bum | - |
dc.contributor.author | Choi, Du Young | - |
dc.contributor.author | In, Kwang-Ho | - |
dc.contributor.author | Kim, Sung-Woo | - |
dc.contributor.author | Park, Hyun | - |
dc.date.accessioned | 2021-09-06T11:53:00Z | - |
dc.date.available | 2021-09-06T11:53:00Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2012-12-28 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/106631 | - |
dc.description.abstract | It is clinically important to be able to detect influenza A/H1N1 virus using a fast, portable, and accurate system that has high specificity and sensitivity. To achieve this goal, it is necessary to develop a highly specific primer set that recognizes only influenza A viral genes and a rapid real-time PCR system that can detect even a single copy of the viral gene. In this study, we developed and validated a novel fluidic chip-type real-time PCR (LabChip real-time PCR) system that is sensitive and specific for the detection of influenza A/H1N1, including the pandemic influenza strain A/H1N1 of 2009. This LabChip real-time PCR system has several remarkable features: (1) It allows rapid quantitative analysis, requiring only 15 min to perform 30 cycles of real-time PCR. (2) It is portable, with a weight of only 5.5 kg. (3) The reaction cost is low, since it uses disposable plastic chips. (4) Its high efficiency is equivalent to that of commercially available tube-type real-time PCR systems. The developed disposable LabChip is an economic, heat-transferable, light-transparent, and easy-to-fabricate polymeric chip compared to conventional silicon- or glass-based labchip. In addition, our LabChip has large surface-to-volume ratios in micro channels that are required for overcoming time consumed for temperature control during real-time PCR. The efficiency of the LabChip real-time PCR system was confirmed using novel primer sets specifically targeted to the hemagglutinin (HA) gene of influenza A/H1N1 and clinical specimens. Eighty-five human clinical swab samples were tested using the LabChip real-time PCR. The results demonstrated 100% sensitivity and specificity, showing 72 positive and 13 negative cases. These results were identical to those from a tube-type real-time PCR system. This indicates that the novel LabChip real-time PCR may be an ultra-fast, quantitative, point-of-care-potential diagnostic tool for influenza A/H1N1 with a high sensitivity and specificity. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | A H1N1 VIRUS | - |
dc.subject | SWINE-ORIGIN 2009 | - |
dc.subject | NUCLEIC-ACID AMPLIFICATION | - |
dc.subject | RT-PCR | - |
dc.subject | MICROFLUIDIC DEVICES | - |
dc.subject | SENSITIVITY | - |
dc.subject | TESTS | - |
dc.subject | TRANSMISSION | - |
dc.subject | PATHOGENESIS | - |
dc.subject | PERFORMANCE | - |
dc.title | Polymeric LabChip Real-Time PCR as a Point-of-Care-Potential Diagnostic Tool for Rapid Detection of Influenza A/H1N1 Virus in Human Clinical Specimens | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | In, Kwang-Ho | - |
dc.identifier.doi | 10.1371/journal.pone.0053325 | - |
dc.identifier.scopusid | 2-s2.0-84871673851 | - |
dc.identifier.wosid | 000313051500135 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.7, no.12 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 7 | - |
dc.citation.number | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | A H1N1 VIRUS | - |
dc.subject.keywordPlus | SWINE-ORIGIN 2009 | - |
dc.subject.keywordPlus | NUCLEIC-ACID AMPLIFICATION | - |
dc.subject.keywordPlus | RT-PCR | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICES | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordPlus | TESTS | - |
dc.subject.keywordPlus | TRANSMISSION | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | PERFORMANCE | - |
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