Functional 3D Human Primary Hepatocyte Spheroids Made by Co-Culturing Hepatocytes from Partial Hepatectomy Specimens and Human Adipose-Derived Stem Cells
DC Field | Value | Language |
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dc.contributor.author | No, Da Yoon | - |
dc.contributor.author | Lee, Seung-A | - |
dc.contributor.author | Choi, Yoon Young | - |
dc.contributor.author | Park, DoYeun | - |
dc.contributor.author | Jang, Ju Yun | - |
dc.contributor.author | Kim, Dong-Sik | - |
dc.contributor.author | Lee, Sang-Hoon | - |
dc.date.accessioned | 2021-09-06T12:02:49Z | - |
dc.date.available | 2021-09-06T12:02:49Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2012-12-07 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/106669 | - |
dc.description.abstract | We have generated human hepatocyte spheroids with uniform size and shape by co-culturing 1:1 mixtures of primary human hepatocytes (hHeps) from partial hepatectomy specimens and human adipose-derived stem cells (hADSCs) in concave microwells. The hADSCs in spheroids could compensate for the low viability and improve the functional maintenance of hHeps. Co-cultured spheroids aggregated and formed compact spheroidal shapes more rapidly, and with a significantly higher viability than mono-cultured spheroids. The liver-specific functions of co-cultured spheroids were greater, although they contained half the number of hepatocytes as mono-cultured spheroids. Albumin secretion by co-cultured spheroids was 10% higher on day 7, whereas urea secretion was similar, compared with mono-cultured spheroids. A quantitative cytochrome P450 assay showed that the enzymatic activity of co-cultured spheroids cultured for 9 days was 28% higher than that of mono-cultured spheroids. These effects may be due to the transdifferentiation potential and paracrine healing effects of hADSCs on hHeps. These co-cultured spheroids may be useful for creating artificial three-dimensional hepatic tissue constructs and for cell therapy with limited numbers of human hepatocytes. Citation: No DY, Lee S-A, Choi YY, Park D, Jang JY, et al. (2012) Functional 3D Human Primary Hepatocyte Spheroids Made by Co-Culturing Hepatocytes from Partial Hepatectomy Specimens and Human Adipose-Derived Stem Cells. PLoS ONE 7(12): e50723. doi:10.1371/journal.pone.0050723 | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | IN-VITRO | - |
dc.subject | HEPATIC LINEAGE | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | LIVER | - |
dc.subject | TISSUE | - |
dc.subject | SIZE | - |
dc.subject | SCAFFOLDS | - |
dc.subject | VIVO | - |
dc.title | Functional 3D Human Primary Hepatocyte Spheroids Made by Co-Culturing Hepatocytes from Partial Hepatectomy Specimens and Human Adipose-Derived Stem Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Hoon | - |
dc.identifier.doi | 10.1371/journal.pone.0050723 | - |
dc.identifier.scopusid | 2-s2.0-84870954967 | - |
dc.identifier.wosid | 000312064100029 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.7, no.12 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 7 | - |
dc.citation.number | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | HEPATIC LINEAGE | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | SIZE | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | VIVO | - |
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