Isolation and Identification of Fungi from a Meju Contaminated with Aflatoxins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Yu Jung | - |
dc.contributor.author | Chung, Soo Hyun | - |
dc.contributor.author | Lee, Hyo Ku | - |
dc.contributor.author | Chun, Hyang Sook | - |
dc.contributor.author | Hong, Seung Beom | - |
dc.date.accessioned | 2021-09-06T12:22:53Z | - |
dc.date.available | 2021-09-06T12:22:53Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2012-12 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/106743 | - |
dc.description.abstract | A home-made meju sample contaminated naturally with aflatoxins was used for isolation of fungal strains. Overall, 230 fungal isolates were obtained on dichloran rosebengal chloramphenicol (DRBC) and dichloran 18% glycerol (DG18) agar plates. Morphological characteristics and molecular analysis of a partial beta-tubulin gene and the internal transcribed spacer (ITS) of rDNA were used for the identification of the isolates. The fungal isolates were divided into 7 genera: Aspergillus, Eurotium, Penicillium, Eupenicillium, Mucor, Lichtheimia, and Curvularia. Three strains from 56 isolates of the A. oryzae/flavus group were found to be aflatoxigenic A. flavus, by the presence of the aflatoxin biosynthesis genes and confirmatory aflatoxin production by high-performance liquid chromatography (HPLC). The predominant isolate from DRBC plates was A. oryzae (42 strains, 36.2%), whereas that from DG18 was A. candidus (61 strains, 53.5%). Out of the 230 isolates, the most common species was A. candidus (34.3%) followed by A. oryzae (22.2%), Mucor circinelloides (13.0%), P. polonicum (10.0%), A. tubingensis (4.8%), and L. ramosa (3.5%). A. flavus and E. chevalieri presented occurrence levels of 2.2%, respectively. The remaining isolates of A. unguis, R oxalicum, Eupenicillium cinnamopurpureum, A. acidus, E. rubrum, P. chrysogenum, M. racemosus, and C. inaequalis had lower occurrence levels of < 2.0%. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | BIOSYNTHESIS GENE-CLUSTER | - |
dc.subject | ASPERGILLUS-ORYZAE | - |
dc.subject | PARASITICUS | - |
dc.subject | STRAINS | - |
dc.subject | ASSAY | - |
dc.subject | DNA | - |
dc.title | Isolation and Identification of Fungi from a Meju Contaminated with Aflatoxins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Soo Hyun | - |
dc.identifier.doi | 10.4014/jmb.1207.07048 | - |
dc.identifier.scopusid | 2-s2.0-84871144663 | - |
dc.identifier.wosid | 000313088500019 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.22, no.12, pp.1740 - 1748 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 22 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1740 | - |
dc.citation.endPage | 1748 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001721566 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | BIOSYNTHESIS GENE-CLUSTER | - |
dc.subject.keywordPlus | ASPERGILLUS-ORYZAE | - |
dc.subject.keywordPlus | PARASITICUS | - |
dc.subject.keywordPlus | STRAINS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | Meju | - |
dc.subject.keywordAuthor | fungi | - |
dc.subject.keywordAuthor | aflatoxigenicity | - |
dc.subject.keywordAuthor | fungal frequency | - |
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