Coenzyme Q10 increases the fatty acid oxidation through AMPK-mediated PPAR alpha induction in 3T3-L1 preadipocytes
- Authors
- Lee, Soo Kyung; Lee, Jung Ok; Kim, Ji Hae; Kim, Nami; You, Ga Young; Moon, Ji Wook; Sha, Jie; Kim, Su Jin; Lee, Yong Woo; Kang, Ho Jin; Park, Sun Hwa; Kim, Hyeon Soo
- Issue Date
- 12월-2012
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Adipogenesis; AMPK; Coenzyme Q10; Fatty acid oxidation; PPAR alpha
- Citation
- CELLULAR SIGNALLING, v.24, no.12, pp.2329 - 2336
- Indexed
- SCIE
SCOPUS
- Journal Title
- CELLULAR SIGNALLING
- Volume
- 24
- Number
- 12
- Start Page
- 2329
- End Page
- 2336
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/106795
- DOI
- 10.1016/j.cellsig.2012.07.022
- ISSN
- 0898-6568
- Abstract
- Coenzyme Q10(CoQ10) is a known anti-adipogenic factor. However, the mechanism by which CoQ10 acts is unclear. In this study, we found that CoQ10 increased the phosphorylation of AMP-activated protein kinase (AMPK) in 3T3-L1preadipocytes. CoQ10 induced an increase in cytoplasmic calcium concentrations, which is reflected by increased Fluo-3 intensity under confocal microscopy recording. Either inhibition of Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) or knock-down CaMKK blocked CoQ10-induced AMPK phosphorylation, suggesting the involvement of calcium in CoQ10-mediated AMPK signaling. CoQ10 also increased the expression of peroxisome proliferator-activated receptor alpha (PPAR alpha) at both the mRNA and protein levels. Knock down of AMPK with siRNA or inhibition of AMPK using an AMPK inhibitor compound C blocked CoQ10-induced expression of PPAR alpha, indicating that AMPK plays a critical role in PPAR alpha induction. In addition, CoQ10 increased fatty acid oxidation in 3T3-L1preadipocytes. The promoter activity of PPAR alpha was increased by CoQ10 in an AMPK-dependent fashion. Moreover, the induction of acyl-CoA oxidase (ACO), a target gene of PPAR alpha, was blocked under the PPAR alpha knock down condition. Furthermore, treatment with CoQ10 blocked differentiation-induced adipogenesis. This blockade was not observed under the PPAR alpha knock-down condition. Collectively, these results demonstrate that CoQ10 induces PPAR alpha expression via the calcium-mediated AMPK signal pathway and suppresses differentiation-induced adipogenesis. (c) 2012 Elsevier Inc. All rights reserved.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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