BLT2 Up-Regulates Interleukin-8 Production and Promotes the Invasiveness of Breast Cancer Cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyunju | - |
dc.contributor.author | Choi, Jung-A | - |
dc.contributor.author | Park, Geun-Soo | - |
dc.contributor.author | Kim, Jae-Hong | - |
dc.date.accessioned | 2021-09-06T13:16:10Z | - |
dc.date.available | 2021-09-06T13:16:10Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2012-11-07 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/106951 | - |
dc.description.abstract | Background: The elevated production of interleukin (IL)-8 is critically associated with invasiveness and metastatic potential in breast cancer cells. However, the intracellular signaling pathway responsible for up-regulation of IL-8 production in breast cancer cells has remained unclear. Methodology/Principal Findings: In this study, we report that the expression of BLT2 is markedly up-regulated in the highly aggressive human breast cancer cell lines MDA-MB-231 and MDA-MB-435 compared with MCF-10A immortalized human mammary epithelial cells, as determined by RT-PCR, real-time PCR and FACS analysis. Blockade of BLT2 with BLT2 siRNA knockdown or BLT2 inhibitor treatment downregulated IL-8 production and thereby diminished the invasiveness of aggressive breast cancer cells, analyzed by Matrigel invasion chamber assays. We further characterized the downstream signaling mechanism by which BLT2 stimulates IL-8 production and identified critical mediatory roles for the generation of reactive oxygen species (ROS) and the consequent activation of the transcription factor NF-kappa B. Moreover, blockade of BLT2 suppressed the formation of metastatic lung nodules by MDA-MB-231 cells in both experimental and orthotopic metastasis models. Conclusions/Significance: Taken together, our study demonstrates that a BLT2-ROS-NF-kappa B pathway up-regulates IL-8 production in MDA-MB-231 and MDA-MB-435 cells, thereby contributing to the invasiveness of these aggressive breast cancer cells. Our findings provide insight into the molecular mechanism of invasiveness in breast cancer. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | LEUKOTRIENE B-4 RECEPTOR | - |
dc.subject | INDEPENDENT GROWTH | - |
dc.subject | PANCREATIC-CANCER | - |
dc.subject | CARCINOMA CELLS | - |
dc.subject | OVARIAN-CANCER | - |
dc.subject | METASTASIS | - |
dc.subject | PATHWAY | - |
dc.subject | EXPRESSION | - |
dc.subject | TRANSCRIPTION | - |
dc.title | BLT2 Up-Regulates Interleukin-8 Production and Promotes the Invasiveness of Breast Cancer Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jae-Hong | - |
dc.identifier.doi | 10.1371/journal.pone.0049186 | - |
dc.identifier.scopusid | 2-s2.0-84868683141 | - |
dc.identifier.wosid | 000311935800227 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.7, no.11 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 7 | - |
dc.citation.number | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | LEUKOTRIENE B-4 RECEPTOR | - |
dc.subject.keywordPlus | INDEPENDENT GROWTH | - |
dc.subject.keywordPlus | PANCREATIC-CANCER | - |
dc.subject.keywordPlus | CARCINOMA CELLS | - |
dc.subject.keywordPlus | OVARIAN-CANCER | - |
dc.subject.keywordPlus | METASTASIS | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
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