Structures of the ribosome-inactivating protein from barley seeds reveal a unique activation mechanism
DC Field | Value | Language |
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dc.contributor.author | Lee, Byung-Gil | - |
dc.contributor.author | Kim, Min Kyung | - |
dc.contributor.author | Kim, Byeong-Won | - |
dc.contributor.author | Suh, Se Won | - |
dc.contributor.author | Song, Hyun Kyu | - |
dc.date.accessioned | 2021-09-06T13:56:45Z | - |
dc.date.available | 2021-09-06T13:56:45Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2012-11 | - |
dc.identifier.issn | 2059-7983 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/107108 | - |
dc.description.abstract | Ribosome-inactivating protein (RIP), a defence protein found in various plants, possesses different chain architectures and activation mechanisms. The RIP from barley (bRIP) is a type I RIP and has sequence features that are divergent from those of type I and type II RIPs from dicotyledonous plants and even the type III RIP from maize. This study presents the first crystal structure of an RIP from a cereal crop, barley, in free, AMP-bound and adenine-bound states. For phasing, a codon-optimized synthetic brip1 gene was used and a vector was constructed to overexpress soluble bRIP fusion proteins; such expression has been verified in a number of cases. The overall structure of bRIP shows folding similar to that observed in other RIPs but also shows significant differences in specific regions, particularly in a switch region that undergoes a structural transition between a 3(10)-helix and a loop depending on the liganded state. The switch region is in a position equivalent to that of a proteolytically susceptible and putative ribosome-binding site in type III RIPs. Thus, the bRIP structure confirms the detailed enzymatic mechanism of this N-glycosidase and reveals a novel activation mechanism for type I RIPs from cereal crops. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | INT UNION CRYSTALLOGRAPHY | - |
dc.subject | RICIN A-CHAIN | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | INHIBITOR | - |
dc.subject | CRYSTALLIZATION | - |
dc.subject | IMMUNOTOXINS | - |
dc.subject | SERVER | - |
dc.subject | PLANTS | - |
dc.subject | TOXIN | - |
dc.subject | SITE | - |
dc.title | Structures of the ribosome-inactivating protein from barley seeds reveal a unique activation mechanism | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Hyun Kyu | - |
dc.identifier.doi | 10.1107/S0907444912037110 | - |
dc.identifier.scopusid | 2-s2.0-84867787918 | - |
dc.identifier.wosid | 000310069500007 | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, v.68, pp.1488 - 1500 | - |
dc.relation.isPartOf | ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | - |
dc.citation.volume | 68 | - |
dc.citation.startPage | 1488 | - |
dc.citation.endPage | 1500 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Crystallography | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.subject.keywordPlus | RICIN A-CHAIN | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | CRYSTALLIZATION | - |
dc.subject.keywordPlus | IMMUNOTOXINS | - |
dc.subject.keywordPlus | SERVER | - |
dc.subject.keywordPlus | PLANTS | - |
dc.subject.keywordPlus | TOXIN | - |
dc.subject.keywordPlus | SITE | - |
dc.subject.keywordAuthor | barley | - |
dc.subject.keywordAuthor | cereal crops | - |
dc.subject.keywordAuthor | monocots | - |
dc.subject.keywordAuthor | RIP | - |
dc.subject.keywordAuthor | surface-entropy reduction | - |
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