SILKWORM 30K PROTEIN INHIBITS ECDYSONE-INDUCED APOPTOSIS BY BLOCKING THE BINDING OF ULTRASPIRACLE TO ECDYSONE RECEPTOR-B1 IN CULTURED Bm5 CELLS
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Mi Young | - |
dc.contributor.author | Song, Hwa Young | - |
dc.contributor.author | Kim, Jin Hee | - |
dc.contributor.author | Kim, Bo Yong | - |
dc.contributor.author | Park, Sun Whan | - |
dc.contributor.author | Sung, Dong Kyung | - |
dc.contributor.author | Park, Hun Hee | - |
dc.contributor.author | Jeon, Soung Hoo | - |
dc.contributor.author | Chung, In Sik | - |
dc.contributor.author | Lee, Bong Hee | - |
dc.date.accessioned | 2021-09-06T13:59:43Z | - |
dc.date.available | 2021-09-06T13:59:43Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2012-11 | - |
dc.identifier.issn | 0739-4462 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/107125 | - |
dc.description.abstract | This study investigates the mechanism through which increased 30K protein inhibits ecdysone-induced apoptosis in the Bm5 silkworm ovarian cell line. Treatment of Bm5 cells with 20-hydroxyecdysone (20E) after transfection with the pIZT/V5-His control vector triggered apoptosis, but 20E treatment did not trigger apoptosis in Bm5 cells transfected with the pIZT/30K/V5-His vector. To confirm its inhibitory effect on apoptosis, 30K protein was first purified from Escherichia coli transformed with a 30K expression vector and used to generate specific antibodies in mice. Anti-30K antiserum was used to confirm synthesis of the 30K protein in pIZT/30K/V5-His-transfected Bm5 cells and to detect 30K protein binding to the ecdysone receptor-B1 (EcR-B1). Anti-30K antiserum was used to immunoprecipitate protein complexes containing 30K from Bm5 cells transfected with pIZT/30K/V5-His vector and treated with 20E. We observed that 30K proteins bound primarily to the EcR-B1 and not to ultraspiracle (USP). Reciprocal immunoprecipitation of EcR-B1-containing complexes from Bm5 cells transfected with control pIZT/V5-His vector and treated with 20E showed that EcR-B1 bound to USP in the absence of 30K but did not bind to USP in pIZT/30K/V5-His-transfected Bm5 cells. These results demonstrate that 30K proteins block USP binding to EcR-B1 through formation of a 30K/EcR-B1 complex, resulting in inhibition of 20E-induced Bm5 cell apoptosis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | MORI LEPIDOPTERA BOMBYCIDAE | - |
dc.subject | MAJOR PLASMA-PROTEINS | - |
dc.subject | BOMBYX-MORI | - |
dc.subject | DROSOPHILA ULTRASPIRACLE | - |
dc.subject | MOLECULAR-PROPERTIES | - |
dc.subject | DEATH | - |
dc.subject | HEMOLYMPH | - |
dc.subject | GLAND | - |
dc.subject | ECR | - |
dc.subject | METAMORPHOSIS | - |
dc.title | SILKWORM 30K PROTEIN INHIBITS ECDYSONE-INDUCED APOPTOSIS BY BLOCKING THE BINDING OF ULTRASPIRACLE TO ECDYSONE RECEPTOR-B1 IN CULTURED Bm5 CELLS | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Bong Hee | - |
dc.identifier.doi | 10.1002/arch.21050 | - |
dc.identifier.scopusid | 2-s2.0-84867636230 | - |
dc.identifier.wosid | 000309926800003 | - |
dc.identifier.bibliographicCitation | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, v.81, no.3, pp.136 - 147 | - |
dc.relation.isPartOf | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | - |
dc.citation.title | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | - |
dc.citation.volume | 81 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 136 | - |
dc.citation.endPage | 147 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Entomology | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Entomology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.subject.keywordPlus | MORI LEPIDOPTERA BOMBYCIDAE | - |
dc.subject.keywordPlus | MAJOR PLASMA-PROTEINS | - |
dc.subject.keywordPlus | BOMBYX-MORI | - |
dc.subject.keywordPlus | DROSOPHILA ULTRASPIRACLE | - |
dc.subject.keywordPlus | MOLECULAR-PROPERTIES | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | HEMOLYMPH | - |
dc.subject.keywordPlus | GLAND | - |
dc.subject.keywordPlus | ECR | - |
dc.subject.keywordPlus | METAMORPHOSIS | - |
dc.subject.keywordAuthor | 30K protein | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | Bm5 cells | - |
dc.subject.keywordAuthor | (ecdysone) | - |
dc.subject.keywordAuthor | silkworm | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.