Inflammation Modulates Murine Venous Thrombosis Resolution In Vivo Assessment by Multimodal Fluorescence Molecular Imaging
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ripplinger, Crystal M. | - |
dc.contributor.author | Kessinger, Chase W. | - |
dc.contributor.author | Li, Chunqiang | - |
dc.contributor.author | Kim, Jin Won | - |
dc.contributor.author | McCarthy, Jason R. | - |
dc.contributor.author | Weissleder, Ralph | - |
dc.contributor.author | Henke, Peter K. | - |
dc.contributor.author | Lin, Charles P. | - |
dc.contributor.author | Jaffer, Farouc A. | - |
dc.date.accessioned | 2021-09-06T14:04:09Z | - |
dc.date.available | 2021-09-06T14:04:09Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2012-11 | - |
dc.identifier.issn | 1079-5642 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/107149 | - |
dc.description.abstract | Objective-Assessment of thrombus inflammation in vivo could provide new insights into deep vein thrombosis (DVT) resolution. Here, we develop and evaluate 2 integrated fluorescence molecular-structural imaging strategies to quantify DVT-related inflammation and architecture and to assess the effect of thrombus inflammation on subsequent DVT resolution in vivo. Methods and Results-Murine DVT were created with topical 5% FeCl3 application to thigh or jugular veins (n=35). On day 3, mice received macrophage and matrix metalloproteinase activity fluorescence imaging agents. On day 4, integrated assessment of DVT inflammation and architecture was performed using confocal fluorescence intravital microscopy. Day 4 analyses showed robust relationships among in vivo thrombus macrophages, matrix metalloproteinase activity, and fluorescein isothiocyanate-dextran deposition (r>0.70; P<0.01). In a serial 2-time point study, mice with DVT underwent intravital microscopy at day 4 and day 6. Analyses revealed that the intensity of thrombus inflammation at day 4 predicted the magnitude of DVT resolution at day 6 (P<0.05). In a second approach, noninvasive fluorescence molecular tomography-computed tomography was used and detected macrophages within jugular DVT (P<0.05 versus sham controls). Conclusion-Integrated fluorescence molecular-structural imaging demonstrates that the DVT-induced inflammatory response can be readily assessed in vivo and can inform the magnitude of thrombus resolution. (Arterioscler Thromb Vasc Biol. 2012;32:2616-2624.) | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | - |
dc.subject | DEEP-VEIN THROMBOSIS | - |
dc.subject | POSTTHROMBOTIC SYNDROME | - |
dc.subject | MOUSE MODEL | - |
dc.subject | CARDIOVASCULAR-DISEASE | - |
dc.subject | ATHEROSCLEROSIS | - |
dc.subject | PLASMINOGEN | - |
dc.subject | RECRUITMENT | - |
dc.subject | MICROSCOPY | - |
dc.subject | LEUKOCYTES | - |
dc.subject | RAT | - |
dc.title | Inflammation Modulates Murine Venous Thrombosis Resolution In Vivo Assessment by Multimodal Fluorescence Molecular Imaging | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jin Won | - |
dc.identifier.doi | 10.1161/ATVBAHA.112.251983 | - |
dc.identifier.scopusid | 2-s2.0-84871886732 | - |
dc.identifier.wosid | 000310058900014 | - |
dc.identifier.bibliographicCitation | ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, v.32, no.11, pp.2616 - + | - |
dc.relation.isPartOf | ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | - |
dc.citation.title | ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | - |
dc.citation.volume | 32 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 2616 | - |
dc.citation.endPage | + | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Cardiovascular System & Cardiology | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Peripheral Vascular Disease | - |
dc.subject.keywordPlus | DEEP-VEIN THROMBOSIS | - |
dc.subject.keywordPlus | POSTTHROMBOTIC SYNDROME | - |
dc.subject.keywordPlus | MOUSE MODEL | - |
dc.subject.keywordPlus | CARDIOVASCULAR-DISEASE | - |
dc.subject.keywordPlus | ATHEROSCLEROSIS | - |
dc.subject.keywordPlus | PLASMINOGEN | - |
dc.subject.keywordPlus | RECRUITMENT | - |
dc.subject.keywordPlus | MICROSCOPY | - |
dc.subject.keywordPlus | LEUKOCYTES | - |
dc.subject.keywordPlus | RAT | - |
dc.subject.keywordAuthor | deep vein thrombosis | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | macrophage | - |
dc.subject.keywordAuthor | molecular imaging | - |
dc.subject.keywordAuthor | post-thrombotic syndrome | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.