A manganese superoxide dismutase (MnSOD) from Ruditapes philippinarum: Comparative structural- and expressional-analysis with copper/zinc superoxide dismutase (Cu/ZnSOD) and biochemical analysis of its antioxidant activities
- Authors
- Umasuthan, Navaneethaiyer; Bathige, S. D. N. K.; Revathy, Kasthuri Saranya; Lee, Youngdeuk; Whang, Ilson; Choi, Cheol Young; Park, Hae-Chul; Lee, Jehee
- Issue Date
- 10월-2012
- Publisher
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
- Keywords
- Ruditapes philippinarum; Manganese superoxide dismutase (MnSOD); Copper/znic superoxide dimutase (Cu/ZnSOD); Gene expression; Antioxidant activity
- Citation
- FISH & SHELLFISH IMMUNOLOGY, v.33, no.4, pp.753 - 765
- Indexed
- SCIE
SCOPUS
- Journal Title
- FISH & SHELLFISH IMMUNOLOGY
- Volume
- 33
- Number
- 4
- Start Page
- 753
- End Page
- 765
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/107261
- DOI
- 10.1016/j.fsi.2012.06.024
- ISSN
- 1050-4648
- Abstract
- Superoxide dismutases (SODs), antioxidant metalloenzymes, represent the first line of defense in biological systems against oxidative stress caused by excessive reactive oxygen species (ROS), in particular O-2(center dot-) Two distinct members of SOD family were identified from Manila clam Ruditapes philippinarum (abbreviated as RpMnSOD and RpCu/ZnSOD). The structural analysis revealed all common characteristics of SOD family in both RpSODs from primary to tertiary levels, including three MnSOD signatures and two Cu/ZnSOD signatures as well as invariant Mn2+- and Cugn(2+)-binding sites in RpMnSOD and RpCu/ZnSOD, respectively. Putative RpMnSOD and RpCu/ZnSOD proteins were predicted to be localized in mitochondrial matrix and cytosol, respectively. They shared 65.2% and 63.9% of identity with human MnSOD and Cu/ZnSOD, respectively. Phylogentic evidences indicated the emergence of RpSODs within molluscan monophyletic dale. The analogous spatial expression profiles of RpSODs demonstrated their higher mRNA levels in hemocytes and gills. The experimental challenges with poly I:C, lipopolysaccharide and Vibrio tapetis illustrated the time-dependent dynamic expression of RpSODs in hemocytes and gills. The recombinant RpMnSOD was expressed in a prokaryotic system and its antioxidant property was studied. The rRpMnSOD exhibited its optimum activity at 20 degrees C, under alkaline condition (pH 9) with a specific activity of 3299 U mg(-1). These outcomes suggested that RpSODs were constitutively expressing inducible proteins that might play crucial role(s) in innate immunity of Manila clam. (C) 2012 Elsevier Ltd. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.