Rapid and sensitive detection of Nampt (PBEF/visfatin) in human serum using an ssDNA aptamer-based capacitive biosensor
DC Field | Value | Language |
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dc.contributor.author | Park, Jee-Woong | - |
dc.contributor.author | Kallempudi, Sreenivasa Saravan | - |
dc.contributor.author | Niazi, Javed H. | - |
dc.contributor.author | Gurbuz, Yasar | - |
dc.contributor.author | Youn, Byung-Soo | - |
dc.contributor.author | Gu, Man Bock | - |
dc.date.accessioned | 2021-09-06T14:48:42Z | - |
dc.date.available | 2021-09-06T14:48:42Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2012-10 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/107269 | - |
dc.description.abstract | A single-stranded DNA (ssDNA) aptamer was successfully developed to specifically bind to nicotinamide phosphoribosyl transferase (Nampt) through systematic evolution of ligands by exponential enrichment (SELEX) and successfully implemented in a gold-interdigitated (GID) capacitor-based biosensor. Surface plasmon resonance (SPR) analysis of the aptamer revealed high specificity and affinity (K-d = 72.52 nM). Changes in surface capacitance/charge distribution or dielectric properties in the response of the GID capacitor surface covalently coupled to the aptamers in response to changes in applied AC frequency were measured as a sensing signal based on a specific interaction between the aptamers and Nampt. The limit of detection for Nampt was 1 ng/ml with a dynamic serum detection range of up to 50 ng/ml; this range includes the clinical requirement for both normal Nampt level, which is 15.8 ng/ml, and Nampt level in type 2 diabetes mellitus (T2DM) patients, which is 31.9 ng/ml. Additionally, the binding kinetics of aptamer-Nampt interactions on the capacitor surface showed that strong binding occurred with increasing frequency (range, 700 MHz-1 GHz) and that the dissociation constant of the aptamer under the applied frequency was improved 120-240 times (K-d = 0.3-0.6 nM) independent on frequency. This assay system is an alternative approach for clinical detection of Nampt with improved specificity and affinity. (C) 2012 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | VISFATIN | - |
dc.subject | CANCER | - |
dc.subject | NAMPT/PBEF/VISFATIN | - |
dc.subject | BIOMARKERS | - |
dc.subject | ENRICHMENT | - |
dc.subject | RESISTIN | - |
dc.subject | MARKERS | - |
dc.subject | LEVEL | - |
dc.subject | ARRAY | - |
dc.title | Rapid and sensitive detection of Nampt (PBEF/visfatin) in human serum using an ssDNA aptamer-based capacitive biosensor | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gu, Man Bock | - |
dc.identifier.doi | 10.1016/j.bios.2012.05.036 | - |
dc.identifier.scopusid | 2-s2.0-84864393537 | - |
dc.identifier.wosid | 000308455700037 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.38, no.1, pp.233 - 238 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 38 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 233 | - |
dc.citation.endPage | 238 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | VISFATIN | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | NAMPT/PBEF/VISFATIN | - |
dc.subject.keywordPlus | BIOMARKERS | - |
dc.subject.keywordPlus | ENRICHMENT | - |
dc.subject.keywordPlus | RESISTIN | - |
dc.subject.keywordPlus | MARKERS | - |
dc.subject.keywordPlus | LEVEL | - |
dc.subject.keywordPlus | ARRAY | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | Nampt (PBEF/visfatin) | - |
dc.subject.keywordAuthor | SELEX | - |
dc.subject.keywordAuthor | Capacitive biosensor | - |
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