Alternative splicing and expression analysis of High expression of osmotically responsive genes1 (HOS1) in Arabidopsis
- Authors
- Lee, Jeong Hwan; Kim, Soo Hyun; Kim, Jae Joon; Ahn, Ji Hoon
- Issue Date
- 30-9월-2012
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- Keywords
- AAB87130; Alternative splicing; BT005517; HOS1; Post-transcriptional regulation
- Citation
- BMB REPORTS, v.45, no.9, pp.515 - 520
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BMB REPORTS
- Volume
- 45
- Number
- 9
- Start Page
- 515
- End Page
- 520
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/107427
- DOI
- 10.5483/BMBRep.2012.45.9.092
- ISSN
- 1976-6696
- Abstract
- High expression of osmotically responsive genes1 (HOS1), a key regulator of low temperature response and flowering time, encodes an E3 ubiquitin ligase in Arabidopsis. Here, we report characterization of a newly identified splice variant (HOS1-L) of HOS1. Comparative analyses revealed that HOS1-L has a longer 5' nucleotide sequence than that of the previously identified HOS1 (HOS1-S) and that its protein sequence was more conserved than that of HOS1-S in plants. HOS1-L transcripts were spatio-temporally more abundant than those of HOS1-S. The recovery rate of HOS1-S expression was faster than that of HOS1-L after cold treatment. Diurnal oscillation patterns of HOS1-L revealed that HOS1-L expression was affected by photoperiod. An in vitro pull-down assay revealed that the HOS1-L protein interacted with the ICE1 protein. HOS1-L overexpression caused delayed flowering in wild-type plants. Collectively, these results suggest regulation of HOS1 expression at the post-transcriptional level. [BMB Reports 2012; 45(9): 515-520]
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