ROCK suppression promotes differentiation and expansion of endothelial cells from embryonic stem cell-derived Flk1(+) mesodermal precursor cells
DC Field | Value | Language |
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dc.contributor.author | Joo, Hyung Joon | - |
dc.contributor.author | Choi, Dong-Kyu | - |
dc.contributor.author | Lim, Joon Seo | - |
dc.contributor.author | Park, Jin-Sung | - |
dc.contributor.author | Lee, Seung-Hun | - |
dc.contributor.author | Song, Sukhyun | - |
dc.contributor.author | Shin, Jennifer H. | - |
dc.contributor.author | Lim, Do-Sun | - |
dc.contributor.author | Kim, Injune | - |
dc.contributor.author | Hwang, Ki-Chul | - |
dc.contributor.author | Koh, Gou Young | - |
dc.date.accessioned | 2021-09-06T15:16:08Z | - |
dc.date.available | 2021-09-06T15:16:08Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2012-09-27 | - |
dc.identifier.issn | 0006-4971 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/107429 | - |
dc.description.abstract | Successful differentiation and expansion of endothelial cells (ECs) from embryonic stem cell (ESC)-derived Flk1(+) mesodermal precursor cells (MPCs) requires supplementation of vascular endothelial growth factor-A (VEGF-A). While analyzing VEGF-A/VEGFR2 downstream signaling pathway that underlies the VEGF-A-induced differentiation and expansion of ECs, we fortuitously found that Rho-associated protein kinase (ROCK) inhibitor Y27632 profoundly promoted the differentiation and expansion of ECs from Flk1(+) MPCs while reducing the differentiation and expansion of mural cells. The ROCK suppression-induced expansion of ECs appears to have resulted from promotion of proliferation of ECs via activation of PI3-kinase-Akt signaling. The ECs obtained by the combination of ROCK suppression and VEGF-A supplementation faithfully expressed most pan-EC surface makers, and phenotypic analyses revealed that they were differentiated toward arterial EC. Further incubation of the ICAM2(+) ECs with Y27632 and VEGF-A for 2 days promoted expansion of ECs by 6.5-fold compared with those incubated with only VEGF-A. Importantly, the ROCK suppression- induced ECs displayed neovasculogenic abilities in vitro and in vivo. Thus, supplementation of ROCK inhibitor Y27632 along with VEGF-A in 2D Matrigel culture system provides a simple, efficient, and versatile method for obtaining ample amount of ESC-derived ECs at high purity suitable for use in therapeutic neovascularization. (Blood. 2012;120(13):2733-2744) | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC HEMATOLOGY | - |
dc.subject | VASCULAR PROGENITORS | - |
dc.subject | IN-VITRO | - |
dc.subject | SPROUTING ANGIOGENESIS | - |
dc.subject | MICROFLUIDIC PLATFORM | - |
dc.subject | VEGF | - |
dc.subject | PATHWAY | - |
dc.subject | VASCULOGENESIS | - |
dc.subject | ARTERIAL | - |
dc.subject | KINASES | - |
dc.subject | PROTEIN | - |
dc.title | ROCK suppression promotes differentiation and expansion of endothelial cells from embryonic stem cell-derived Flk1(+) mesodermal precursor cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Joo, Hyung Joon | - |
dc.identifier.doi | 10.1182/blood-2012-04-421610 | - |
dc.identifier.scopusid | 2-s2.0-84866865798 | - |
dc.identifier.wosid | 000311615800029 | - |
dc.identifier.bibliographicCitation | BLOOD, v.120, no.13, pp.2733 - 2744 | - |
dc.relation.isPartOf | BLOOD | - |
dc.citation.title | BLOOD | - |
dc.citation.volume | 120 | - |
dc.citation.number | 13 | - |
dc.citation.startPage | 2733 | - |
dc.citation.endPage | 2744 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.subject.keywordPlus | VASCULAR PROGENITORS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | SPROUTING ANGIOGENESIS | - |
dc.subject.keywordPlus | MICROFLUIDIC PLATFORM | - |
dc.subject.keywordPlus | VEGF | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | VASCULOGENESIS | - |
dc.subject.keywordPlus | ARTERIAL | - |
dc.subject.keywordPlus | KINASES | - |
dc.subject.keywordPlus | PROTEIN | - |
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