Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles
- Authors
- Yoo, Myung-Ik; Seo, Yeong-Ju; Choi, Kyu-Sil; Ha, Jeong Sook; Woo, Kyoungja
- Issue Date
- 1-9월-2012
- Publisher
- ELSEVIER
- Keywords
- Superparamagnetic iron oxide nanoparticles (SPIONs); Scatteredly cRCDfK-conjugated; Ultrasmall; Water-dispersible; Lipophilic; Cellular uptake
- Citation
- COLLOIDS AND SURFACES B-BIOINTERFACES, v.97, pp.175 - 181
- Indexed
- SCIE
SCOPUS
- Journal Title
- COLLOIDS AND SURFACES B-BIOINTERFACES
- Volume
- 97
- Start Page
- 175
- End Page
- 181
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/107481
- DOI
- 10.1016/j.colsurfb.2012.04.009
- ISSN
- 0927-7765
- Abstract
- We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin alpha(v)beta(3)) and MCF-7 cells (human breast cancer cells, express low level of integrin alpha(v)beta(3)) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs(SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin alpha(v)beta(3)-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs. (c) 2012 Elsevier B.V. All rights reserved.
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