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Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC

Authors
Jung, JuYu, Kyung OkRamzi, Ahmad BazliChoe, Se HoonKim, Seung WookHan, Sung Ok
Issue Date
9월-2012
Publisher
WILEY
Keywords
Surfactin; Bacillus subtilis; Signaling peptides; Synthetic wastewater
Citation
BIOTECHNOLOGY AND BIOENGINEERING, v.109, no.9, pp.2349 - 2356
Indexed
SCIE
SCOPUS
Journal Title
BIOTECHNOLOGY AND BIOENGINEERING
Volume
109
Number
9
Start Page
2349
End Page
2356
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/107578
DOI
10.1002/bit.24524
ISSN
0006-3592
Abstract
Surfactin is a biological surfactant with numerous potential applications. In this study, Bacillus subtilis was engineered to improve surfactin production by the activation of two competence-stimulating pheromones, ComX and competence and sporulation factor (CSF) to stimulate the transcription of srfA operon. Both signaling factors, encoded by comX and phrC, were successfully overexpressed and subsequently increased surfactin production. Surfactin produced by engineered strains showed functional groups similar to the commercially available surfactin analyzed via Fourier transform infrared spectroscopy (FTIR). Surfactin production in the B. subtilis (pHT43-comXphrC) strain was 6.4-fold greater than in the wild strain, with approximately 135.1?mg/L surfactin produced after 48?h cultivation. To reduce the production costs of surfactin, synthetic wastewater was used, from which the B. subtilis (pHT43-comXphrC) strain produced approximately 140.2?mg/L surfactin. The results obtained demonstrated the production of surfactin from synthetic wastewater, which is beneficial in lowering the overall production costs. Biotechnol. Bioeng. 2012;109: 23492356. (c) 2012 Wiley Periodicals, Inc.
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College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
Graduate School > Department of Biotechnology > 1. Journal Articles

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