Increased expression level and catalytic activity of internally-duplicated carbonic anhydrase from Dunaliella species by reconstitution of two separate domains
DC Field | Value | Language |
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dc.contributor.author | Ki, Mi-Ran | - |
dc.contributor.author | Kanth, Bashistha Kumar | - |
dc.contributor.author | Min, Ki Ha | - |
dc.contributor.author | Lee, Jinwon | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.date.accessioned | 2021-09-06T16:07:11Z | - |
dc.date.available | 2021-09-06T16:07:11Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/107588 | - |
dc.description.abstract | Although internally-duplicated, alpha-type carbonic anhydrase (CA) from Dunaliella species (Dsp-CA) can be expressed in Escherichia coli system, the produced amount is not sufficient for practical application. Here, we demonstrated to express the N- and C-half CA domains of Dsp-CA as distinct proteins to increase the expression levels further. The purified N-half CA domain (Dsp-CA-n) showed very low detectable activities of esterase or CO2 hydratase. In contrast, the purified C-half CA domain (Dsp-CA-c) retained both activities, which were enhanced by the presence of Dsp-CA-n. The expression levels of both domains were increased by 2-5-fold as compared to that of Dsp-CA. The CA activity was successfully reconstituted by mixing the two domains, N- and C-half domains, and more activity level was achieved than dimeric intact CA. These results newly suggest that the produced amount and activity of a duplicated CA are enhanced successfully by expressing each half CA domain individually and in vitro reconstitution. (C) 2012 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | BIOMIMETIC SEQUESTRATION | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | CO2 | - |
dc.subject | PRECIPITATION | - |
dc.subject | MORPHOLOGY | - |
dc.subject | CALCITE | - |
dc.subject | ACETATE | - |
dc.subject | SALINA | - |
dc.subject | SALT | - |
dc.title | Increased expression level and catalytic activity of internally-duplicated carbonic anhydrase from Dunaliella species by reconstitution of two separate domains | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ki, Mi-Ran | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1016/j.procbio.2012.05.005 | - |
dc.identifier.scopusid | 2-s2.0-84862875051 | - |
dc.identifier.wosid | 000306777400018 | - |
dc.identifier.bibliographicCitation | PROCESS BIOCHEMISTRY, v.47, no.9, pp.1423 - 1427 | - |
dc.relation.isPartOf | PROCESS BIOCHEMISTRY | - |
dc.citation.title | PROCESS BIOCHEMISTRY | - |
dc.citation.volume | 47 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1423 | - |
dc.citation.endPage | 1427 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | BIOMIMETIC SEQUESTRATION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CO2 | - |
dc.subject.keywordPlus | PRECIPITATION | - |
dc.subject.keywordPlus | MORPHOLOGY | - |
dc.subject.keywordPlus | CALCITE | - |
dc.subject.keywordPlus | ACETATE | - |
dc.subject.keywordPlus | SALINA | - |
dc.subject.keywordPlus | SALT | - |
dc.subject.keywordAuthor | Carbonic anhydrase | - |
dc.subject.keywordAuthor | Dunaliella species | - |
dc.subject.keywordAuthor | Repeat domain | - |
dc.subject.keywordAuthor | CO2 mineralization | - |
dc.subject.keywordAuthor | Calcite | - |
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