Enhanced 2,3-Butanediol Production in Recombinant Klebsiella pneumoniae via Overexpression of Synthesis-Related Genes
DC Field | Value | Language |
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dc.contributor.author | Kim, Borim | - |
dc.contributor.author | Lee, Soojin | - |
dc.contributor.author | Park, Joohong | - |
dc.contributor.author | Lu, Mingshou | - |
dc.contributor.author | Oh, Minkyu | - |
dc.contributor.author | Kim, Youngrok | - |
dc.contributor.author | Lee, Jinwon | - |
dc.date.accessioned | 2021-09-06T16:09:14Z | - |
dc.date.available | 2021-09-06T16:09:14Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/107600 | - |
dc.description.abstract | 2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2,3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2,3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2,3-BD production level achieved through fed-batch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2,3-BD synthesis-related genes can enhance 2,3-BD production in K. pneumoniae by fermentation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | FERMENTATION PATHWAY | - |
dc.subject | STATE | - |
dc.title | Enhanced 2,3-Butanediol Production in Recombinant Klebsiella pneumoniae via Overexpression of Synthesis-Related Genes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Minkyu | - |
dc.identifier.doi | 10.4014/jmb.1201.01044 | - |
dc.identifier.scopusid | 2-s2.0-84864096711 | - |
dc.identifier.wosid | 000309305400013 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.22, no.9, pp.1258 - 1263 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 22 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1258 | - |
dc.citation.endPage | 1263 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001696427 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | FERMENTATION PATHWAY | - |
dc.subject.keywordPlus | STATE | - |
dc.subject.keywordAuthor | 2,3-Butanediol | - |
dc.subject.keywordAuthor | Klebsiella pneumoniae | - |
dc.subject.keywordAuthor | gene manipulation | - |
dc.subject.keywordAuthor | acetolactate decarboxylase | - |
dc.subject.keywordAuthor | acetolactate synthase | - |
dc.subject.keywordAuthor | alcohol dehydrogenase | - |
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