Synthesis and characterization of glucose oxidase-core/shell magnetic nanoparticle complexes into chitosan bead
DC Field | Value | Language |
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dc.contributor.author | Lee, Hee Uk | - |
dc.contributor.author | Song, Yoon Seok | - |
dc.contributor.author | Suh, Young Joon | - |
dc.contributor.author | Park, Chulhwan | - |
dc.contributor.author | Kim, Seung Wook | - |
dc.date.accessioned | 2021-09-06T16:12:32Z | - |
dc.date.available | 2021-09-06T16:12:32Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.issn | 1381-1177 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/107619 | - |
dc.description.abstract | The goal of this study was to improve the activity and stability of glucose oxidase (GOD) immobilized on Co-B/SiO2 nanoparticles (NPs) entrapped in chitosan beads. The Co-B/SiO2 NPs were prepared from a silica shell-coated Co-B core using the Stober method. GOD was covalently immobilized on the surface of Co-B/SiO2/NH2 NPs using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) as an activating agent. The optimal conditions for immobilization of GOD onto chitosan bead (IGCB) were a 1.2 wt% of chitosan solution, and 2.0 wt% of sodium tripolyphosphate (TPP). K-M and V-max were determined to be 60.7 mM and 43.5 mu M/min for free GODs (FG), 15.2 mM and 4.8 mu M/min for immobilized GODs (IG), and 51.2 mM and 5.0 mu M/min for IGCB, respectively. After being entrapped into the chitosan beads, the GOD exhibited improved storage and operation stability. The IC and IGCB retained 48% and 64% of their initial activity after 7 reuses, respectively. (c) 2012 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | MICROSPHERES | - |
dc.subject | PERFORMANCE | - |
dc.subject | PARTICLES | - |
dc.title | Synthesis and characterization of glucose oxidase-core/shell magnetic nanoparticle complexes into chitosan bead | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Seung Wook | - |
dc.identifier.doi | 10.1016/j.molcatb.2012.05.004 | - |
dc.identifier.scopusid | 2-s2.0-84861669159 | - |
dc.identifier.wosid | 000306577100006 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v.81, pp.31 - 36 | - |
dc.relation.isPartOf | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | - |
dc.citation.title | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | - |
dc.citation.volume | 81 | - |
dc.citation.startPage | 31 | - |
dc.citation.endPage | 36 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | MICROSPHERES | - |
dc.subject.keywordPlus | PERFORMANCE | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordAuthor | Co-B/SiO2 | - |
dc.subject.keywordAuthor | Core/shell | - |
dc.subject.keywordAuthor | Immobilization | - |
dc.subject.keywordAuthor | Glucose oxidase | - |
dc.subject.keywordAuthor | Chitosan bead | - |
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