Therapeutic potential of mesenchymal stem cells overexpressing human forkhead box A2 gene in the regeneration of damaged liver tissues
DC Field | Value | Language |
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dc.contributor.author | Cho, Jong-Woo | - |
dc.contributor.author | Lee, Chul-Young | - |
dc.contributor.author | Ko, Yong | - |
dc.date.accessioned | 2021-09-06T17:23:07Z | - |
dc.date.available | 2021-09-06T17:23:07Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-08 | - |
dc.identifier.issn | 0815-9319 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/107855 | - |
dc.description.abstract | Background and Aim: Although a liver transplantation is considered to be the only effective long-term treatment in many cases of liver diseases, it is limited by a lack of donor organs and immune rejection. As an autologous stem cell approach, this study was conducted to assess whether forkhead box A2 (Foxa2) gene overexpression in bone marrow-derived mesenchymal stem cells (MSC) could protect the liver from hepatic diseases by stimulating tissue regeneration after cell transplantation. Methods: Rat MSC (rMSC) were isolated, characterized, and induced to hepatocytes that expressed liver-specific markers. Four different treatments (control [phosphate-buffered saline], rMSC alone, rMSC/pIRESenhanced green fluorescent protein (EGFP) vector, and rMSC/pIRESEGFP/human Foxa2) were injected into the spleen of carbon tetrachloride-injured rats. Biochemical and histological analyses on days 30, 60, and 90 post-transplantation were performed to evaluate the therapeutic capacities of MSC overexpressing hFoxa2. Results: rMSC transfected with hFoxa2 were induced into hepatogenic linage and expressed several liver-specific genes, such as, Foxa2, a-fetoprotein, cytokeratin-18, hepatocyte nuclear factor-1a, and hepatocyte growth factor. A group of animals treated with MSC/hFoxa2 showed significant recovery of liver-specific enzyme expressions to normal levels at the end of the study (90 days). Furthermore, when compared to the fibrotic areas of the samples treated with MSC alone or MSC/vector, the fibrotic area of the samples treated with rMSC/hFoxa2 for 90 days significantly decreased, until they were completely gone. Conclusions: Human Foxa2 efficiently promoted the incorporation of MSC into liver grafts, suggesting that hFoxa2 genes could be used for the structural or functional recovery of damaged liver cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | TRANSCRIPTION FACTORS | - |
dc.subject | HEPATIC DIFFERENTIATION | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | MARROW | - |
dc.subject | FIBROSIS | - |
dc.subject | HEPATOCYTES | - |
dc.subject | MOUSE | - |
dc.subject | MICE | - |
dc.subject | FOXA | - |
dc.subject | RATS | - |
dc.title | Therapeutic potential of mesenchymal stem cells overexpressing human forkhead box A2 gene in the regeneration of damaged liver tissues | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ko, Yong | - |
dc.identifier.doi | 10.1111/j.1440-1746.2012.07137.x | - |
dc.identifier.scopusid | 2-s2.0-84864314102 | - |
dc.identifier.wosid | 000306650700018 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, v.27, no.8, pp.1362 - 1370 | - |
dc.relation.isPartOf | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.citation.title | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.citation.volume | 27 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1362 | - |
dc.citation.endPage | 1370 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTORS | - |
dc.subject.keywordPlus | HEPATIC DIFFERENTIATION | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | MARROW | - |
dc.subject.keywordPlus | FIBROSIS | - |
dc.subject.keywordPlus | HEPATOCYTES | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | FOXA | - |
dc.subject.keywordPlus | RATS | - |
dc.subject.keywordAuthor | forkhead box A2 gene | - |
dc.subject.keywordAuthor | hepatocyte | - |
dc.subject.keywordAuthor | mesenchymal stem cell | - |
dc.subject.keywordAuthor | stem cell therapy | - |
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