Electrochemical sensor based on Au nanoparticles decorated boron-doped diamond electrode using ferrocene-tagged aptamer for proton detection
- Authors
- Song, Min-Jung; Lee, Seung-Koo; Lee, Jun-Yong; Kim, Jong-Hoon; Lim, Dae-Soon
- Issue Date
- 15-7월-2012
- Publisher
- ELSEVIER SCIENCE SA
- Keywords
- Proton; Ferrocene; i-Motif structure; Aptamer sensor; Boron-doped diamond; pH
- Citation
- JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v.677, pp.139 - 144
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF ELECTROANALYTICAL CHEMISTRY
- Volume
- 677
- Start Page
- 139
- End Page
- 144
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/107927
- DOI
- 10.1016/j.jelechem.2012.05.019
- ISSN
- 1572-6657
- Abstract
- An electrochemical sensor based on a boron-doped diamond (BDD) electrode decorated with gold nanoparticles (Au-NPs) was developed for the detection of protons using a ferrocene-tagged (Fc-tagged) aptamer. By introducing the Au-NPs, the aptamer with a thiol-terminated group was immobilized on the Au-NPs/BDD electrode by Au-S interaction. Morphology and characteristics of the modified BDD electrode were analyzed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The employed aptamer is a cytosine-rich single stranded oligonucleotide. In this oligonucleotide, the distance between the labeled redox moiety and the electrode changes with the pH value because of the formation of an i-motif structure from cytosine-protonated cytosine (C-C+) pairs. The pH influenced the electron transfer process and the redox current. The synergetic effects of the BDD electrode and the Au-NPs improved the performance of this sensor. Therefore, the Au-NPs/BDD electrode exhibited higher sensitivity, faster response, a wider linear range and a good reproducibility for the detection of proton compared with the planar Au electrode. (C) 2012 Elsevier B.V. All rights reserved.
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