Production of cellulases and beta-glucosidase in Trichoderma reesei mutated by proton beam irradiation
DC Field | Value | Language |
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dc.contributor.author | Jung, You Ree | - |
dc.contributor.author | Shin, Hyun Yong | - |
dc.contributor.author | Yoo, Hah Young | - |
dc.contributor.author | Um, Youngsoon | - |
dc.contributor.author | Kim, Seung Wook | - |
dc.date.accessioned | 2021-09-06T18:02:20Z | - |
dc.date.available | 2021-09-06T18:02:20Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-07 | - |
dc.identifier.issn | 0256-1115 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/108013 | - |
dc.description.abstract | To obtain mutant strains producing high levels of cellulases (FPase and CMCase) and beta-glucosidase, Trichoderma reesei KCTC 6950 was mutated by proton beam irradiation. Five mutants were selected out of 1,000 mutants of T.reesei treated with proton beam irradiation, based on their ability for enzyme production on a plate screening medium. In submerged cultures containing Mandel's fermentation medium, the mutant strain T-2 (MT-2) demonstrated a 165% increase in the activity of FPase, a 146% increase in the activity of CMCase, and a 313% increase in the activity of beta-glucosidase, compared with the wild type strain. Additionally, the properties of high level beta-glucosidase produced by MT-2 were the same as those of the wild type strain, e.g., an optimum pH of 4.8, and an optimum temperature of 65 A degrees C. Moreover, the protein concentrations of beta-glucosidase produced by the wild type strain and MT-2 were measured by SDS-PAGE, and then beta-glucosidase activities were detected by the MUG-zymogram assay. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN INSTITUTE CHEMICAL ENGINEERS | - |
dc.subject | ENZYMATIC-HYDROLYSIS | - |
dc.subject | ENHANCED PRODUCTION | - |
dc.subject | STRAIN IMPROVEMENT | - |
dc.subject | PURIFICATION | - |
dc.subject | SELECTION | - |
dc.subject | VIRIDE | - |
dc.title | Production of cellulases and beta-glucosidase in Trichoderma reesei mutated by proton beam irradiation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Seung Wook | - |
dc.identifier.doi | 10.1007/s11814-011-0272-5 | - |
dc.identifier.scopusid | 2-s2.0-84863568120 | - |
dc.identifier.wosid | 000305959300013 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.29, no.7, pp.925 - 930 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF CHEMICAL ENGINEERING | - |
dc.citation.title | KOREAN JOURNAL OF CHEMICAL ENGINEERING | - |
dc.citation.volume | 29 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 925 | - |
dc.citation.endPage | 930 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001669682 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | ENZYMATIC-HYDROLYSIS | - |
dc.subject.keywordPlus | ENHANCED PRODUCTION | - |
dc.subject.keywordPlus | STRAIN IMPROVEMENT | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | VIRIDE | - |
dc.subject.keywordAuthor | Cellulases | - |
dc.subject.keywordAuthor | beta-Glucosidase | - |
dc.subject.keywordAuthor | Trichoderma reesei | - |
dc.subject.keywordAuthor | Proton Beam Irradiation | - |
dc.subject.keywordAuthor | Mutation | - |
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