12(S)-Hydroxyheptadeca-5Z,8E,10E-trienoic acid suppresses UV-induced IL-6 synthesis in keratinocytes, exerting an anti-inflammatory activity
DC Field | Value | Language |
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dc.contributor.author | Lee, Jin-Wook | - |
dc.contributor.author | Ryu, Ho-Cheol | - |
dc.contributor.author | Ng, Yee Ching | - |
dc.contributor.author | Kim, Cheolmin | - |
dc.contributor.author | Wei, Jun-Dong | - |
dc.contributor.author | Sabaratnam, Vikineswary | - |
dc.contributor.author | Kim, Jae-Hong | - |
dc.date.accessioned | 2021-09-06T18:28:18Z | - |
dc.date.available | 2021-09-06T18:28:18Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-06-30 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/108130 | - |
dc.description.abstract | 12(5)-Hydroxyheptadeca-5Z,8E,10E-trienoic acid (12-HHT) is an enzymatic product of prostaglandin H-2 (PGH(2)) derived from cyclooxygenase (COX)-mediated arachidonic acid metabolism. Despite the high level of 12-HHT present in tissues and bodily fluids, its precise function remains largely unknown. In this study, we found that 12-HHT treatment in HaCaT cells remarkably down-regulated the ultraviolet B (UVB) irradiation-induced synthesis of interleukin-6 (IL-6), a pro-inflammatory cytokine associated with cutaneous inflammation. In an approach to identify the down-stream signaling mechanism by which 12-HHT down-regulates UVB-induced IL-6 synthesis in keratinocytes, we observed that 12-HHT inhibits the UVB-stimulated activation of p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-kappa B). In addition, we found that 12-HHT markedly up-regulates MAPK phosphatase-1 (MKP-1), a critical negative regulator of p38 MAPK. When MKP-1 was suppressed by siRNA knock-down, the 12-HHT-mediated inhibitory effects on the UVB-stimulated activation of p38 MAPK and NF-kappa B, as well as the production of IL-6, were attenuated in HaCaT cells. Taken together, our results suggest that 12-HHT exerts anti-inflammatory effect via up-regulation of MKP-1, which negatively regulates p38 MAPK and NF-kappa b, thus attenuating IL-6 production in UVB-irradiated HaCaT cells. Considering the critical role of IL-6 in cutaneous inflammation, our findings provide the basis for the application of 12-HHT as a potential anti-inflammatory therapeutic agent in UV-induced skin diseases. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY | - |
dc.subject | ULTRAVIOLET-B IRRADIATION | - |
dc.subject | CULTURED HUMAN KERATINOCYTES | - |
dc.subject | P38 MAP KINASE | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | IMMUNE-RESPONSE | - |
dc.subject | UP-REGULATION | - |
dc.subject | C-JUN | - |
dc.subject | ACTIVATION | - |
dc.subject | SKIN | - |
dc.subject | INDUCTION | - |
dc.title | 12(S)-Hydroxyheptadeca-5Z,8E,10E-trienoic acid suppresses UV-induced IL-6 synthesis in keratinocytes, exerting an anti-inflammatory activity | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jae-Hong | - |
dc.identifier.doi | 10.3858/emm.2012.44.6.043 | - |
dc.identifier.scopusid | 2-s2.0-84863446062 | - |
dc.identifier.wosid | 000305922100003 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.44, no.6, pp.378 - 386 | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 44 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 378 | - |
dc.citation.endPage | 386 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001668807 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | ULTRAVIOLET-B IRRADIATION | - |
dc.subject.keywordPlus | CULTURED HUMAN KERATINOCYTES | - |
dc.subject.keywordPlus | P38 MAP KINASE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | IMMUNE-RESPONSE | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | C-JUN | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SKIN | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordAuthor | dermatitis | - |
dc.subject.keywordAuthor | dual specificity phosphatase 1 | - |
dc.subject.keywordAuthor | 12-hydroxy-5,8,10-heptadecatrienoic acid | - |
dc.subject.keywordAuthor | interleukin-6 | - |
dc.subject.keywordAuthor | ultraviolet rays | - |
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