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Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays

Authors
Lee, Su JinPark, Jee-WoongKim, In-AeYoun, Byung-SooGu, Man Bock
Issue Date
20-6월-2012
Publisher
ELSEVIER SCIENCE SA
Keywords
Aptarners; Adipokines; Antibodies; ELAAS biosensor; Type 2 diabetes
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v.168, pp.243 - 248
Indexed
SCIE
SCOPUS
Journal Title
SENSORS AND ACTUATORS B-CHEMICAL
Volume
168
Start Page
243
End Page
248
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/108145
DOI
10.1016/j.snb.2012.04.016
ISSN
0925-4005
Abstract
We developed an enzyme-linked antibody-ssDNA aptamer sandwich (ELAAS) method to detect three adipokines for diagnosing type 2 diabetes. For this application, three different aptamers for retinol-binding protein-4 (RBP4), visceral adipose tissue-derived serpin (Vaspin), and nicotina mide phosphoribosyltransferase (Nampt/visfatin) were used as capturing probes, and enzyme-linked polyclonal antibodies were used for signal generation. The sensitivities of this method for all three protein:; were enhanced by at least 20-fold to up to 68-fold higher than that of the surface plasmon resonance (S PR) system, which only uses aptamers as capturing probes. In addition, this assay method exhibited equal sensitivity in the detection of each adipokine spiked in diluted human serum. In particular, the detection limit for RBP4 was similar to that obtained by western blotting, as reported previously. The simplicity, reproducibility, and high sensitivity of the ELAAS method could allow it to be extensively utilized in field. (C) 2012 Elsevier B.V. All rights reserved.
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