The natural carotenoid astaxanthin, a PPAR-alpha agonist and PPAR-gamma antagonist, reduces hepatic lipid accumulation by rewiring the transcriptome in lipid-loaded hepatocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jia, Yaoyao | - |
dc.contributor.author | Kim, Jin-Young | - |
dc.contributor.author | Jun, Hee-Jin | - |
dc.contributor.author | Kim, Sun-Joong | - |
dc.contributor.author | Lee, Ji-Hae | - |
dc.contributor.author | Hoang, Minh Hien | - |
dc.contributor.author | Hwang, Kwang-Yeon | - |
dc.contributor.author | Um, Soo-Jong | - |
dc.contributor.author | Chang, Hyo Ihl | - |
dc.contributor.author | Lee, Sung-Joon | - |
dc.date.accessioned | 2021-09-06T19:04:24Z | - |
dc.date.available | 2021-09-06T19:04:24Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 1613-4125 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/108229 | - |
dc.description.abstract | Scope: A natural carotenoid abundant in seafood, astaxanthin (AX), has hypolipidemic activity, but its underlying mechanisms of action and protein targets are unknown. We investigated the molecular mechanism of action of AX in hepatic hyperlipidemia by measuring peroxisome proliferator-activated receptors (PPAR) activity. Methods and results: We examined the binding of AX to PPAR subtypes and its effects on hepatic lipid metabolism. AX binding activated PPAR-alpha, but inhibited PPAR-gamma transactivation activity in reporter gene assay and time-resolved fluorescence energy transfer analyses. AX had no effect on PPAR delta/beta transactivation. AX bound directly to PPAR-alpha and PPAR-gamma with moderate affinity, as assessed by surface plasmon resonance experiments. The differential effects of AX on PPARs were confirmed by measuring the expression of unique responsive genes for each PPAR subtype. AX significantly reduced cellular lipid accumulation in lipid-loaded hepatocytes. Transcriptome analysis revealed that the net effects of stimulation with AX (100 mu M) on lipid metabolic pathways were similar to those elicited by fenofibrate and lovastatin (10 mu M each), with AX rewiring the expression of genes involved in lipid metabolic pathways. Conclusion: AX is a PPAR-alpha agonist and PPAR-gamma antagonist, reduces hepatic lipid accumulation by rewiring the transcriptome in lipid-loaded hepatocytes. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | DUAL AGONISTS | - |
dc.subject | ACID | - |
dc.subject | INFLAMMATION | - |
dc.subject | METABOLISM | - |
dc.subject | ATHEROSCLEROSIS | - |
dc.subject | STEATOSIS | - |
dc.subject | RECEPTORS | - |
dc.subject | DELTA | - |
dc.subject | MICE | - |
dc.title | The natural carotenoid astaxanthin, a PPAR-alpha agonist and PPAR-gamma antagonist, reduces hepatic lipid accumulation by rewiring the transcriptome in lipid-loaded hepatocytes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Kwang-Yeon | - |
dc.contributor.affiliatedAuthor | Chang, Hyo Ihl | - |
dc.identifier.doi | 10.1002/mnfr.201100798 | - |
dc.identifier.scopusid | 2-s2.0-84862575244 | - |
dc.identifier.wosid | 000305323100005 | - |
dc.identifier.bibliographicCitation | MOLECULAR NUTRITION & FOOD RESEARCH, v.56, no.6, pp.878 - 888 | - |
dc.relation.isPartOf | MOLECULAR NUTRITION & FOOD RESEARCH | - |
dc.citation.title | MOLECULAR NUTRITION & FOOD RESEARCH | - |
dc.citation.volume | 56 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 878 | - |
dc.citation.endPage | 888 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | DUAL AGONISTS | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | ATHEROSCLEROSIS | - |
dc.subject.keywordPlus | STEATOSIS | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | DELTA | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | Astaxanthin | - |
dc.subject.keywordAuthor | Lipid metabolism | - |
dc.subject.keywordAuthor | PPAR | - |
dc.subject.keywordAuthor | Transcriptome | - |
dc.subject.keywordAuthor | Seafood | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.