Production of functional agarolytic nano-complex for the synergistic hydrolysis of marine biomass and its potential application in carbohydrate-binding module-utilizing one-step purification
- Authors
- Hyeon, Jeong Eun; Kang, Dae Hee; Kim, Young In; Jeon, Sang Duck; You, Seung Kyou; Kim, Kyung Yeon; Kim, Seung Wool; Han, Sung Ok
- Issue Date
- 5월-2012
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Agar; beta-Agarase; Agarolytic complex; Carbohydrate-binding module; Scaffolding protein; Zobellia galactanivorans
- Citation
- PROCESS BIOCHEMISTRY, v.47, no.5, pp.877 - 881
- Indexed
- SCIE
SCOPUS
- Journal Title
- PROCESS BIOCHEMISTRY
- Volume
- 47
- Number
- 5
- Start Page
- 877
- End Page
- 881
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/108511
- DOI
- 10.1016/j.procbio.2012.01.009
- ISSN
- 1359-5113
- Abstract
- Marine algal polysaccharides are promising alternative resources to terrestrial biomass. Agarolytic enzymes degrade agarose to various kinds of oligosaccharides. In this study, we expressed miniCbpA, a recombinant scaffolding protein from Clostridium cellulovorans, in Escherichia coli. To assemble the agarolytic complex via cohesin-dockerin interaction, we constructed a chimeric agarase cAgaB from Zobellia galactanivorans containing the catalytic domain of AgaB fused with a dockerin domain from C. cellulovorans EngB. The assembly of functional agarolytic complexes increased the activity against the agar substrate approximately 1,4-fold compared with that for the corresponding enzymes alone. The carbohydrate-binding module (CBM) of miniCbpA was used as a tag for CBM-utilizing one-step purification using cellulose as a support. This is the first report on the formation of agarolytic complexes using the cohesin-dockerin interaction system. The assembly of agar-degrading complexes will lead to the commercial production of useful products from agar biomass at low costs. (C) 2012 Elsevier Ltd. All rights reserved.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
- Graduate School > Department of Biotechnology > 1. Journal Articles
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