Widdrol induces apoptosis via activation of AMP-activated protein kinase in colon cancer cells
DC Field | Value | Language |
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dc.contributor.author | Kang, Moo Rim | - |
dc.contributor.author | Park, Song-Kyu | - |
dc.contributor.author | Lee, Chang Woo | - |
dc.contributor.author | Choi, Ig Jun | - |
dc.contributor.author | Jo, Yeong Nang | - |
dc.contributor.author | Yang, Jeong Wook | - |
dc.contributor.author | Kim, Jin-Ah | - |
dc.contributor.author | Yun, Jieun | - |
dc.contributor.author | Lee, Ki Hoon | - |
dc.contributor.author | Kwon, Hyun Ju | - |
dc.contributor.author | Kim, Byung Woo | - |
dc.contributor.author | Lee, Kiho | - |
dc.contributor.author | Kang, Jong Soon | - |
dc.contributor.author | Kim, Hwan Mook | - |
dc.date.accessioned | 2021-09-06T20:34:25Z | - |
dc.date.available | 2021-09-06T20:34:25Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-05 | - |
dc.identifier.issn | 1021-335X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/108613 | - |
dc.description.abstract | Widdrol,a natural sesquiterpene present in Juniperus sp., has been shown to exert anticancer and antifungal effects. Emerging evidence has suggested that AMP-activated protein kinase (AMPK), which functions as a cellular energy sensor, is a potential therapeutic target for human cancers. In this study, we found that AMPK mediates the anticancer effects of widdrol through induction of apoptosis in HT-29 colon cancer cells. We showed that widdrol induced the phosphorylation of AMPK in a dose- and time-dependent manner. The selective AMPK inhibitor compound C abrogated the inhibitory effect of widdrol on HT-29 cell growth. In addition, we demonstrated that widdrol induced apoptosis and this was associated with the activation of caspases, including caspase-3/7 and caspase-9, in HT-29 cells. We also demonstrated that transfection of HT-29 cells with AMPK si RNAs significantly suppressed the widdrol-mediated apoptosis and the activation of caspases. However, cell cycle arrest induced by widdrol was not affected by transfection of HT-29 cells with AMPK siRNAs. Furthermore, widdrol inhibited HT-29 tumor growth in a human tumor xenograft model. Taken together, our results suggest that the anticancer effect of widdrol may be mediated, at least in part, by induction of apoptosis via AMPK activation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.subject | PATHWAY | - |
dc.subject | CYCLE | - |
dc.subject | METFORMIN | - |
dc.subject | SIGNAL | - |
dc.subject | VIVO | - |
dc.subject | LKB1 | - |
dc.subject | EGCG | - |
dc.title | Widdrol induces apoptosis via activation of AMP-activated protein kinase in colon cancer cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Song-Kyu | - |
dc.contributor.affiliatedAuthor | Lee, Kiho | - |
dc.identifier.doi | 10.3892/or.2012.1644 | - |
dc.identifier.scopusid | 2-s2.0-84863286988 | - |
dc.identifier.wosid | 000302202600017 | - |
dc.identifier.bibliographicCitation | ONCOLOGY REPORTS, v.27, no.5, pp.1407 - 1412 | - |
dc.relation.isPartOf | ONCOLOGY REPORTS | - |
dc.citation.title | ONCOLOGY REPORTS | - |
dc.citation.volume | 27 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1407 | - |
dc.citation.endPage | 1412 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | CYCLE | - |
dc.subject.keywordPlus | METFORMIN | - |
dc.subject.keywordPlus | SIGNAL | - |
dc.subject.keywordPlus | VIVO | - |
dc.subject.keywordPlus | LKB1 | - |
dc.subject.keywordPlus | EGCG | - |
dc.subject.keywordAuthor | widdrol | - |
dc.subject.keywordAuthor | AMP-activated protein kinase | - |
dc.subject.keywordAuthor | colon cancer | - |
dc.subject.keywordAuthor | apoptosis | - |
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