Requirement of the Cytosolic Interaction between PATHOGENESIS-RELATED PROTEIN10 and LEUCINE-RICH REPEAT PROTEIN1 for Cell Death and Defense Signaling in Pepper
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Du Seok | - |
dc.contributor.author | Hwang, In Sun | - |
dc.contributor.author | Hwang, Byung Kook | - |
dc.date.accessioned | 2021-09-06T21:48:03Z | - |
dc.date.available | 2021-09-06T21:48:03Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-04 | - |
dc.identifier.issn | 1040-4651 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/108846 | - |
dc.description.abstract | Plants recruit innate immune receptors such as leucine-rich repeat (LRR) proteins to recognize pathogen attack and activate defense genes. Here, we identified the pepper (Capsicum annuum) pathogenesis-related protein10 (PR10) as a leucine-rich repeat protein1 (LRR1)-interacting partner. Bimolecular fluorescence complementation and coimmunoprecipitation assays confirmed the specific interaction between LRR1 and PR10 in planta. Avirulent Xanthomonas campestris pv vesicatoria infection induces PR10 expression associated with the hypersensitive cell death response. Transient expression of PR10 triggers hypersensitive cell death in pepper and Nicotiana benthamiana leaves, which is amplified by LRR1 coexpression as a positive regulator. LRR1 promotes the ribonuclease activity and phosphorylation of PR10, leading to enhanced cell death signaling. The LRR1-PR10 complex is formed in the cytoplasm, resulting in its secretion into the apoplastic space. Engineered nuclear confinement of both proteins revealed that the cytoplasmic localization of the PR10-LRR1 complex is essential for cell death-mediated defense signaling. PR10/LRR1 silencing in pepper compromises resistance to avirulent X. campestris pv vesicatoria infection. By contrast, PR10/LRR1 overexpression in Arabidopsis thaliana confers enhanced resistance to Pseudomonas syringae pv tomato and Hyaloperonospora arabidopsidis. Together, these results suggest that the cytosolic LRR-PR10 complex is responsible for cell death-mediated defense signaling. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC PLANT BIOLOGISTS | - |
dc.subject | BIRCH POLLEN ALLERGEN | - |
dc.subject | LESION-MIMIC MUTANT | - |
dc.subject | AGROBACTERIUM-MEDIATED TRANSFORMATION | - |
dc.subject | HYPERSENSITIVE INDUCED REACTION | - |
dc.subject | DISEASE-RESISTANCE | - |
dc.subject | CAPSICUM-ANNUUM | - |
dc.subject | DIFFERENTIAL EXPRESSION | - |
dc.subject | ARABIDOPSIS-THALIANA | - |
dc.subject | MICROBIAL PATHOGENS | - |
dc.subject | SALICYLIC-ACID | - |
dc.title | Requirement of the Cytosolic Interaction between PATHOGENESIS-RELATED PROTEIN10 and LEUCINE-RICH REPEAT PROTEIN1 for Cell Death and Defense Signaling in Pepper | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Byung Kook | - |
dc.identifier.doi | 10.1105/tpc.112.095869 | - |
dc.identifier.scopusid | 2-s2.0-84861709449 | - |
dc.identifier.wosid | 000304595500028 | - |
dc.identifier.bibliographicCitation | PLANT CELL, v.24, no.4, pp.1675 - 1690 | - |
dc.relation.isPartOf | PLANT CELL | - |
dc.citation.title | PLANT CELL | - |
dc.citation.volume | 24 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1675 | - |
dc.citation.endPage | 1690 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | BIRCH POLLEN ALLERGEN | - |
dc.subject.keywordPlus | LESION-MIMIC MUTANT | - |
dc.subject.keywordPlus | AGROBACTERIUM-MEDIATED TRANSFORMATION | - |
dc.subject.keywordPlus | HYPERSENSITIVE INDUCED REACTION | - |
dc.subject.keywordPlus | DISEASE-RESISTANCE | - |
dc.subject.keywordPlus | CAPSICUM-ANNUUM | - |
dc.subject.keywordPlus | DIFFERENTIAL EXPRESSION | - |
dc.subject.keywordPlus | ARABIDOPSIS-THALIANA | - |
dc.subject.keywordPlus | MICROBIAL PATHOGENS | - |
dc.subject.keywordPlus | SALICYLIC-ACID | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.