Toxicological biomarkers of 2,3,4,7,8-pentachlorodibenzofuran in proteins secreted by HepG2 cells
DC Field | Value | Language |
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dc.contributor.author | Phark, Sohee | - |
dc.contributor.author | Park, So-Young | - |
dc.contributor.author | Choi, Seonyoung | - |
dc.contributor.author | Zheng, Zhi | - |
dc.contributor.author | Cho, Eunkyung | - |
dc.contributor.author | Lee, Min | - |
dc.contributor.author | Lim, Ji-youn | - |
dc.contributor.author | Seo, Jong Bok | - |
dc.contributor.author | Won, Nam Hee | - |
dc.contributor.author | Jung, Woon-Won | - |
dc.contributor.author | Sul, Donggeun | - |
dc.date.accessioned | 2021-09-06T21:57:07Z | - |
dc.date.available | 2021-09-06T21:57:07Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-04 | - |
dc.identifier.issn | 1570-9639 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/108897 | - |
dc.description.abstract | Using a proteomic approach, a study was conducted for determination of the effects of 2,3,4,7,8-pentachlorodibenzofuran (2,3,4,7,8-PCDF) on proteins secreted by HepG2 cells. Briefly, HepG2 cells were exposed to various concentrations of 2,3,4,7,8-PCDF for 24 or 48 h. MTT and comet assays were then conducted for determination of cytotoxicity and genotoxicity, respectively. Results of an MTT assay showed that 1 nM of 2,3,4,7,8-PCDF was the maximum concentration that did not cause cell death. In addition, a dose- and time dependent increase of DNA damage was observed in HepG2 cells exposed to 2,3,4,7,8-PCDF. Therefore, two different concentrations of 2,3,4,7,8-PCDF, 1 and 5 nM, were selected for further analysis of proteomic bio-markers using two different pI ranges (4-7 and 6-9) and large two dimensional gel electrophoresis. Results showed identification of 32 proteins ( 29 up- and 3 down-regulated) by nano-LC-ESI-MS/MS and nano-ESI on a Q-TOF2 MS. Among these, the identities of pyridoxine-5'-phosphate oxidase, UDP-glucose 6-dehydrogenase, plasminogen activator inhibitor I precursor, plasminogen activator inhibitor-3, proteasome activator complex subunit 1, isoform 1 of 14-3-3 protein sigma, peptidyl-prolyl cis-trans isomerase A, 14-3-3 protein gamma, protein DJ-1, and nucleoside diphosphate kinase A were confirmed by western blot analysis. The differential expression of protein DJ-1, proteasome activator complex subunit 1 and plasminogen activator inhibitor-3 was further validated in plasma proteins from rats exposed to 2,3,4,7,8-PCDF. These proteins could be used as potential toxicological biomarkers of 2,3,4,7,8-PCDF. (C) 2012 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | UDP-GLUCOSE DEHYDROGENASE | - |
dc.subject | DIBENZO-P-DIOXINS | - |
dc.subject | NUCLEOSIDE-DIPHOSPHATE KINASE | - |
dc.subject | PLASMINOGEN-ACTIVATOR INHIBITOR-1 | - |
dc.subject | PARKINSONS-DISEASE | - |
dc.subject | CANCER-CELLS | - |
dc.subject | POLYCHLORINATED DIBENZOFURANS | - |
dc.subject | PROTEOMIC ANALYSIS | - |
dc.subject | PROSTATE-CANCER | - |
dc.subject | HEPATOMA-CELLS | - |
dc.title | Toxicological biomarkers of 2,3,4,7,8-pentachlorodibenzofuran in proteins secreted by HepG2 cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, So-Young | - |
dc.contributor.affiliatedAuthor | Won, Nam Hee | - |
dc.contributor.affiliatedAuthor | Jung, Woon-Won | - |
dc.contributor.affiliatedAuthor | Sul, Donggeun | - |
dc.identifier.doi | 10.1016/j.bbapap.2012.01.013 | - |
dc.identifier.scopusid | 2-s2.0-84862819033 | - |
dc.identifier.wosid | 000302443000014 | - |
dc.identifier.bibliographicCitation | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v.1824, no.4, pp.656 - 666 | - |
dc.relation.isPartOf | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | - |
dc.citation.title | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | - |
dc.citation.volume | 1824 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 656 | - |
dc.citation.endPage | 666 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | UDP-GLUCOSE DEHYDROGENASE | - |
dc.subject.keywordPlus | DIBENZO-P-DIOXINS | - |
dc.subject.keywordPlus | NUCLEOSIDE-DIPHOSPHATE KINASE | - |
dc.subject.keywordPlus | PLASMINOGEN-ACTIVATOR INHIBITOR-1 | - |
dc.subject.keywordPlus | PARKINSONS-DISEASE | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | POLYCHLORINATED DIBENZOFURANS | - |
dc.subject.keywordPlus | PROTEOMIC ANALYSIS | - |
dc.subject.keywordPlus | PROSTATE-CANCER | - |
dc.subject.keywordPlus | HEPATOMA-CELLS | - |
dc.subject.keywordAuthor | 2,3,4,7,8-pentachlorodibenzofuran | - |
dc.subject.keywordAuthor | Biomarker | - |
dc.subject.keywordAuthor | HepG2 cell | - |
dc.subject.keywordAuthor | Proteomic | - |
dc.subject.keywordAuthor | Rat plasma | - |
dc.subject.keywordAuthor | Two-dimensional gel electrophoresis | - |
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