Adenovirus-mediated heme oxygenase-1 gene transfer to neonatal porcine islet-like cluster cells: the effects on gene expression and protection from cell stress
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yeom, Hye-Jung | - |
dc.contributor.author | Ro, Han | - |
dc.contributor.author | Park, Sol Ji | - |
dc.contributor.author | Hong, Ju Ho | - |
dc.contributor.author | Cho, Bumrae | - |
dc.contributor.author | Kim, Hwajung | - |
dc.contributor.author | Kim, Sung Joo | - |
dc.contributor.author | Hwang, Jong-Ik | - |
dc.contributor.author | Lee, Byeong Chun | - |
dc.contributor.author | Ahn, Curie | - |
dc.contributor.author | Yang, Jaeseok | - |
dc.date.accessioned | 2021-09-06T22:18:46Z | - |
dc.date.available | 2021-09-06T22:18:46Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-03-20 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/108969 | - |
dc.description.abstract | Porcine islet xenotransplantation is a promising strategy for the treatment of diabetes that overcomes donor shortages. However, islet xenografts are susceptible to oxidative stress and apoptosis. Heme oxygenase-1 (HO-1) has been shown to protect cells from oxidative stress, apoptosis and inflammation. Here, we investigated whether introduction of human HO-1 (hHO-1) into neonatal porcine islet-like cell clusters (NPCCs) can induce beneficial transcriptional changes in NPCCs against cellular stress. NPCCs were transduced with either adenovirus-HO-1 (Ad-HO-1) or control adenovirus-GFP (Ad-GFP). After treatment with hydrogen peroxide (H2O2) for 24 hours, nitrite oxide (NO) production assays were performed to detect oxidative stress. Microarray analysis was performed using a pig oligonucleotide 44K gene chip. We profiled transcriptional changes to apoptosis, oxidant and inflammatory genes, and real-time PCR analysis was also performed to confirm the microarray results. Survival of NPCCs after treatment with H2O2 was significantly higher in the Ad-HO-1 group (p<0.001), and NO production also decreased in the Ad-HO-1 group (p<0.01). The microarray results showed that the expression of pro-apoptosis genes such as CASP3, CASP7, CASP10, CIDE-B and CIDE-C was significantly decreased in the Ad-HO-1 virus group (CASP10; p<0.05, CASP3, CIDE-C; p<0.01, CASP7, CIDE-B; p<0.001). We also found that the expression of oxidative stresses genes including COX1, COX2, CYB5A, SDHD and NOS2 was decreased, and that the anti-oxidant genes Gpx1 and SOD2 were increased in the Ad-HO-1 group (NOS2; p<0.05, COX1, COX2, CYB5A, SDHD, SOD2, GPX1; p<0.001). However, inflammatory gene expression was not significantly changed. Real-time PCR analysis confirmed the results of the microarray analysis. These results shed light on the underlying mechanisms of the protective effects of hHO-1 on porcine islets from cellular stresses and suggest that hHO-1 could be a promising target gene for the production of transgenic pigs that confer improved islet xenograft survival. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN BIOCHIP SOCIETY-KBCS | - |
dc.subject | NITRIC-OXIDE | - |
dc.subject | HYDROGEN-PEROXIDE | - |
dc.subject | INDUCED APOPTOSIS | - |
dc.subject | EPITHELIAL-CELLS | - |
dc.subject | GRANZYME-B | - |
dc.subject | IN-VITRO | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | FAMILY | - |
dc.subject | MESSENGER | - |
dc.subject | SYNTHASE | - |
dc.title | Adenovirus-mediated heme oxygenase-1 gene transfer to neonatal porcine islet-like cluster cells: the effects on gene expression and protection from cell stress | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Jong-Ik | - |
dc.identifier.doi | 10.1007/s13206-012-6108-5 | - |
dc.identifier.scopusid | 2-s2.0-84863347356 | - |
dc.identifier.wosid | 000301684500008 | - |
dc.identifier.bibliographicCitation | BIOCHIP JOURNAL, v.6, no.1, pp.56 - 64 | - |
dc.relation.isPartOf | BIOCHIP JOURNAL | - |
dc.citation.title | BIOCHIP JOURNAL | - |
dc.citation.volume | 6 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 56 | - |
dc.citation.endPage | 64 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001642722 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.description.journalRegisteredClass | other | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | GRANZYME-B | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | MESSENGER | - |
dc.subject.keywordPlus | SYNTHASE | - |
dc.subject.keywordAuthor | Hemeoxgenase-1 | - |
dc.subject.keywordAuthor | Adenovirus | - |
dc.subject.keywordAuthor | Neonatal porcine islet-like cell clusters | - |
dc.subject.keywordAuthor | Xenotransplantation | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Oxidative stress | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.