Detection of Nickel in Fish Organs with a Two-Photon Fluorescent Probe
- Authors
- Kang, Min Young; Lim, Chang Su; Kim, Hyun Soo; Seo, Eun Won; Kim, Hwan Myung; Kwon, Ohyun; Cho, Bong Rae
- Issue Date
- 2월-2012
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- fish; fluorescent probes; live tissue; nickel; two-photon microscopy
- Citation
- CHEMISTRY-A EUROPEAN JOURNAL, v.18, no.7, pp.1953 - 1960
- Indexed
- SCIE
SCOPUS
- Journal Title
- CHEMISTRY-A EUROPEAN JOURNAL
- Volume
- 18
- Number
- 7
- Start Page
- 1953
- End Page
- 1960
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/109030
- DOI
- 10.1002/chem.201103191
- ISSN
- 0947-6539
- Abstract
- Molecular imaging by two-photon microscopy (TPM) has become indispensable to the study of biology/medicine owing to its capability of imaging deep inside intact tissues. To make TPM a more-versatile tool, a large variety of two-photon probes are needed. Herein, we report a new two-photon fluorescent probe (ANi2) that can be excited by 750 nm femtosecond pulses and detect Ni2+ ions in fresh fish organs at 90-175 mu m depth without interference from the pH value or from other biologically relevant species through the use of TPM. TPM images of fish organs labeled with ANi2 revealed that Ni2+ ions accumulate in fish organs in the order: kidney > heart > gill >= liver. Moreover, a linear relationship was found between the two-photon-excited fluorescence (TPEF) and the inductively coupled plasma mass spectrometry intensities (ICP-MS), thereby allowing the quantitative measurement of Ni2+ ions in live tissue.
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