Identification and classification of endogenous retroviruses in the canine genome using degenerative PCR and in-silico data analysis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, Haiin | - |
dc.contributor.author | Choi, Hojun | - |
dc.contributor.author | Choi, Min-Kyeung | - |
dc.contributor.author | Song, Ning | - |
dc.contributor.author | Kim, Jin-Hoi | - |
dc.contributor.author | Oh, Jae-Wook | - |
dc.contributor.author | Seo, Kunho | - |
dc.contributor.author | Seo, Han Geuk | - |
dc.contributor.author | Chun, Taehoon | - |
dc.contributor.author | Kim, Tae-Hun | - |
dc.contributor.author | Park, Chankyu | - |
dc.date.accessioned | 2021-09-06T22:58:01Z | - |
dc.date.available | 2021-09-06T22:58:01Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-01-20 | - |
dc.identifier.issn | 0042-6822 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/109066 | - |
dc.description.abstract | Pooled genomic DNA from 10 dogs was subjected to polymerase chain reaction with primers targeting the retroviral pro/pol region. Sequence analysis of 120 clones obtained by PCR revealed 81 of retroviral origin. Subsequent analysis of the dog genome (CanFam 2.0) by BLAST investigation using degenerate PCR products and previously identified retroviral sequences permitted the identification of additional retroviral gamma and beta sequences. A phylogenetic analysis using the retroviral protease (PR) and reverse transcriptase (RT) sequences in the dog genome resulted in identification of 17 gamma and 7 beta families. In addition, we also identified 167 spuma-like ERV elements from CanFam 2.0 based on sequence homology to murine (Mu)ERV-L and human (H)ERV-L. Our results could contribute to the understanding of the influence of retroviruses in shaping the genome structure and altering gene expression by providing quantitative and locational information of ERV loci and their diversity in the dog genome. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | MOLECULAR CLOCK | - |
dc.subject | EVOLUTION | - |
dc.subject | SEQUENCES | - |
dc.subject | DIVERSITY | - |
dc.subject | ELEMENTS | - |
dc.subject | RETROTRANSPOSONS | - |
dc.subject | RETROELEMENTS | - |
dc.subject | DISEASE | - |
dc.subject | ORIGIN | - |
dc.subject | DNA | - |
dc.title | Identification and classification of endogenous retroviruses in the canine genome using degenerative PCR and in-silico data analysis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chun, Taehoon | - |
dc.identifier.doi | 10.1016/j.virol.2011.10.010 | - |
dc.identifier.scopusid | 2-s2.0-84855201422 | - |
dc.identifier.wosid | 000299755900005 | - |
dc.identifier.bibliographicCitation | VIROLOGY, v.422, no.2, pp.195 - 204 | - |
dc.relation.isPartOf | VIROLOGY | - |
dc.citation.title | VIROLOGY | - |
dc.citation.volume | 422 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 195 | - |
dc.citation.endPage | 204 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | ELEMENTS | - |
dc.subject.keywordPlus | RETROTRANSPOSONS | - |
dc.subject.keywordPlus | RETROELEMENTS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | ORIGIN | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | MOLECULAR CLOCK | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.subject.keywordAuthor | Endogenous retrovirus | - |
dc.subject.keywordAuthor | Canine | - |
dc.subject.keywordAuthor | Genome | - |
dc.subject.keywordAuthor | Pro | - |
dc.subject.keywordAuthor | Pol | - |
dc.subject.keywordAuthor | ERV | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.