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Assessing the contribution of cell body and intracellular organelles to the backward light scattering

Authors
Kalashnikov, MaximChoi, WonshikHunter, MartinYu, Chung-ChiehDasari, Ramachandra R.Feld, Michael S.
Issue Date
16-1월-2012
Publisher
OPTICAL SOC AMER
Citation
OPTICS EXPRESS, v.20, no.2, pp.816 - 826
Indexed
SCIE
SCOPUS
Journal Title
OPTICS EXPRESS
Volume
20
Number
2
Start Page
816
End Page
826
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/109074
DOI
10.1364/OE.20.000816
ISSN
1094-4087
Abstract
We report a method of assessing the contribution of whole cell body and its nucleus to the clinically most relevant backward light scattering. We first construct an experimental system that can measure forward scattering and use the system to precisely extract the optical properties of a specimen such as the refractive index contrast, size distribution, and their density. A system that can simultaneously detect the backscattered light is installed to collect the backscattering for the same specimen. By comparing the measured backscattering spectrum with that estimated from the parameters determined by the forward scattering experiment, the contribution of cell body and nucleus to the backward light scattering is quantitatively assessed. For the HeLa cells in suspension, we found that the cell body contributes less than 10% and cell nucleus on the order of 0.1% to the total backscattering signal. Quantitative determination of the origin of backscattered light may help design a system that aims for detecting particular structure of biological tissues. (C)2012 Optical Society of America
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