The soluble amino-terminal region of HVEM mediates efficient herpes simplex virus type 1 infection of gD receptor-negative cells
DC Field | Value | Language |
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dc.contributor.author | Baek, Hyunjung | - |
dc.contributor.author | Kim, Jae Hong | - |
dc.contributor.author | Noh, Yoon Tae | - |
dc.contributor.author | Kwon, Heechung | - |
dc.date.accessioned | 2021-09-06T23:06:09Z | - |
dc.date.available | 2021-09-06T23:06:09Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-01-13 | - |
dc.identifier.issn | 1743-422X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/109082 | - |
dc.description.abstract | Background: Previous studies from our own and other labs reported the surprising finding that the soluble V domain of the herpes simplex virus type 1 (HSV-1) entry receptor nectin-1 can both block HSV infection of receptor-bearing cells and mediate infection of receptor-deficient cells. Here we show that this property is not unique to nectin-1. We generated a pair of truncated, soluble forms of the other major HSV-1 entry receptor, herpes virus entry mediator (HVEM or HveA), and examined its effects on HSV-1 infection of receptor-deficient cells. Results: In cultures of CHO-K1 cells, sHveA(102) comprising the two amino-terminal cysteine-rich pseudorepeats (CRPs) of HVEM enabled infection of greater than 80% of the cells at an MOI of 3, while sHveA(162) comprising the complete ectodomain failed to mediate infection. Both sHveA(102) and sHveA(162) blocked infection of CHO-K1 cells stably expressing HVEM in a dose-dependent manner, indicating that both were capable of binding to viral gD. We found that sHveA(102)-mediated infection involves pH-independent endocytosis whereas HSV infection of HVEM expressing CHO-K1 cells is known to be pH-dependent. Conclusions: Our results suggest that the C-terminal portion of the soluble HVEM ectodomain inhibits gD activation and that this effect is neutralized in the full-length form of HVEM in normal infection. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | 3-O-SULFATED HEPARAN-SULFATE | - |
dc.subject | GLYCOPROTEIN-D | - |
dc.subject | POLIOVIRUS RECEPTOR | - |
dc.subject | DEFICIENT CELLS | - |
dc.subject | LOW PH | - |
dc.subject | ENTRY | - |
dc.subject | BINDING | - |
dc.subject | DOMAIN | - |
dc.subject | MEMBER | - |
dc.subject | ACTIVATION | - |
dc.title | The soluble amino-terminal region of HVEM mediates efficient herpes simplex virus type 1 infection of gD receptor-negative cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jae Hong | - |
dc.identifier.doi | 10.1186/1743-422X-9-15 | - |
dc.identifier.scopusid | 2-s2.0-84855721024 | - |
dc.identifier.wosid | 000304653000001 | - |
dc.identifier.bibliographicCitation | VIROLOGY JOURNAL, v.9 | - |
dc.relation.isPartOf | VIROLOGY JOURNAL | - |
dc.citation.title | VIROLOGY JOURNAL | - |
dc.citation.volume | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.subject.keywordPlus | 3-O-SULFATED HEPARAN-SULFATE | - |
dc.subject.keywordPlus | GLYCOPROTEIN-D | - |
dc.subject.keywordPlus | POLIOVIRUS RECEPTOR | - |
dc.subject.keywordPlus | DEFICIENT CELLS | - |
dc.subject.keywordPlus | LOW PH | - |
dc.subject.keywordPlus | ENTRY | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | MEMBER | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | HSV-1 | - |
dc.subject.keywordAuthor | HVEM/HveA | - |
dc.subject.keywordAuthor | gD | - |
dc.subject.keywordAuthor | Soluble entry receptor | - |
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