Enhanced production of cellobiose dehydrogenase and beta-glucosidase by Phanerochaete chrysosporium

Abstract

The production of cellobiose dehydrogenase (CDH) and beta-glucosidase by Phanerochaete chrysosporium ATCC 32629 was assessed during submerged fermentation. The maximum concentrations of CDH and beta-glucosidase were obtained using rice straw as the carbon source. Organic nitrogen sources were more effective in enzyme production than inorganic nitrogen sources. Corn steep liquor (CSL) for CDH production and soy bean meal (SBM) for beta-glucosidase production were the most appropriate organic nitrogen sources. Using optimum medium obtained by response surface methodology (RSM), the maximum concentrations of CDH and beta-glucosidase achieved in the stirred-tank reactor (STR) were 204 U/L and 140 U/L, respectively. CDH productivity (22.7 U/L.day) was the highest at 9 days.