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A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay
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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Lee, Hye-Jin | - |
| dc.contributor.author | Kim, Byoung Chan | - |
| dc.contributor.author | Oh, Min-Kyu | - |
| dc.contributor.author | Kim, Jungbae | - |
| dc.date.accessioned | 2021-09-06T23:50:08Z | - |
| dc.date.available | 2021-09-06T23:50:08Z | - |
| dc.date.created | 2021-06-18 | - |
| dc.date.issued | 2012 | - |
| dc.identifier.issn | 1359-7345 | - |
| dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/109289 | - |
| dc.description.abstract | Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 degrees C for 66 days. | - |
| dc.language | English | - |
| dc.language.iso | en | - |
| dc.publisher | ROYAL SOC CHEMISTRY | - |
| dc.subject | PROTEIN-DETECTION | - |
| dc.subject | NEGATIVE COOPERATIVITY | - |
| dc.title | A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay | - |
| dc.type | Article | - |
| dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
| dc.contributor.affiliatedAuthor | Kim, Jungbae | - |
| dc.identifier.doi | 10.1039/c2cc30710c | - |
| dc.identifier.scopusid | 2-s2.0-84861632731 | - |
| dc.identifier.wosid | 000304263600003 | - |
| dc.identifier.bibliographicCitation | CHEMICAL COMMUNICATIONS, v.48, no.48, pp.5971 - 5973 | - |
| dc.relation.isPartOf | CHEMICAL COMMUNICATIONS | - |
| dc.citation.title | CHEMICAL COMMUNICATIONS | - |
| dc.citation.volume | 48 | - |
| dc.citation.number | 48 | - |
| dc.citation.startPage | 5971 | - |
| dc.citation.endPage | 5973 | - |
| dc.type.rims | ART | - |
| dc.type.docType | Article | - |
| dc.description.journalClass | 1 | - |
| dc.description.journalRegisteredClass | scie | - |
| dc.description.journalRegisteredClass | scopus | - |
| dc.relation.journalResearchArea | Chemistry | - |
| dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
| dc.subject.keywordPlus | PROTEIN-DETECTION | - |
| dc.subject.keywordPlus | NEGATIVE COOPERATIVITY | - |
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