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Immobilization and stabilization of subtilisin Carlsberg in magnetically-separable mesoporous silica for transesterification in an organic solvent

Authors
Hwang, Ee TaekLee, ByoungsooZhang, MeilingJun, Seung-HyunShim, JongminLee, JinwooKim, JungbaeGu, Man Bock
Issue Date
2012
Publisher
ROYAL SOC CHEMISTRY
Citation
GREEN CHEMISTRY, v.14, no.7, pp.1884 - 1887
Indexed
SCIE
SCOPUS
Journal Title
GREEN CHEMISTRY
Volume
14
Number
7
Start Page
1884
End Page
1887
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/109432
DOI
10.1039/c2gc35559k
ISSN
1463-9262
Abstract
Subtilisin Carlsberg (SC) was immobilized and stabilized on magnetically-separable mesoporous silica (Mag-MSU-F) in the form of nanoscale enzyme reactors (NERs) based on the ship-in-a-bottle mechanism. Stabilized NERs of SC (NER-SC) were freeze-dried and successfully used for the transesterification of N-acetyl-L-phenylalanine ethyl ester with n-propanol in isooctane. Magnetic separation of Mag-MSU-F facilitated the repeated usages of stable NER-SC. This is the first demonstration for the use of stable and magnetically-separable NERs in an organic solvent, which has the potential for environmentally-friendly synthesis using enzymes in organic solvents.
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College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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