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압타머를 이용한 백혈구제거필터의 개발 및 예비평가Development and Preliminary Evaluation of a Leukocyte Removal Aptamer Filter

Other Titles
Development and Preliminary Evaluation of a Leukocyte Removal Aptamer Filter
Authors
이양원정은숙최경영권소영조남선김진숙박한정한병돈윤수영
Issue Date
2012
Publisher
대한수혈학회
Keywords
Aptamer; CD45; Leukocyte reduction
Citation
대한수혈학회지, v.23, no.2, pp.107 - 114
Indexed
KCI
OTHER
Journal Title
대한수혈학회지
Volume
23
Number
2
Start Page
107
End Page
114
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/110313
ISSN
1226-9336
Abstract
Background:Leukocyte reduction filters are widely used to prevent transfusion reactions caused by leukocytes in blood components. Commercial filters are not sufficient for removal of leukocytes for prevention of transfusion associated graft-versus-host disease; therefore, irradiation of blood components was performed using expensive equipment. Techniques using an aptamer substituted for antibody have been developed and are available in clinical areas. The purpose of this study is to develop the aptamer filter system and to evaluate its efficiency and the possibility of its clinical application. Methods:Aptamers targeted to CD45 were selected by the Postech Aptamer Initiative. The aptamer filter in which aptamers attached to beads were bound to leukocytes and removed by magnetic field was developed. Filtration of 14 units of leukoreduction-red blood provided by Korean Red Cross Blood Services was performed using aptamer filters. Leukocyte removal rate and red cell recovery rate were evaluated and bacterial culture was performed. Results:After filtration using the aptamer filters, 45.6% of leukocytes were additionally removed and the red cell recovery rate was 92.8%. No growth in the bacterial culture was observed. Conclusion:In order to apply the cell depletion technique utilizing an aptamer to blood filter system, we developed and evaluated the aptamer filter system. Through improvement of the binding efficiency of the aptamer and the filtering process, and application of the various aptamers for other different cells, we suggest that this technique can be applied in the clinical area, such as a substitution for the irradiation process for TAGVHD prevention
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