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Label-free imaging of membrane potential using membrane electromotility

Authors
Oh, S.Fang-Yen, C.Choi, W.Yaqoob, Z.Fu, D.Park, Y.Dassari, R.R.Feld, M.S.
Issue Date
2012
Citation
Biophysical Journal, v.103, no.1, pp.11 - 18
Indexed
SCIE
SCOPUS
Journal Title
Biophysical Journal
Volume
103
Number
1
Start Page
11
End Page
18
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/110641
DOI
10.1016/j.bpj.2012.05.020
ISSN
0006-3495
Abstract
Electrical activity may cause observable changes in a cell's structure in the absence of exogenous reporter molecules. In this work, we report a low-coherence interferometric microscopy technique that can detect an optical signal correlated with the membrane potential changes in individual mammalian cells without exogenous labels. By measuring milliradian-scale phase shifts in the transmitted light, we can detect changes in the cells' membrane potential. We find that the observed optical signals are due to membrane electromotility, which causes the cells to deform in response to the membrane potential changes. We demonstrate wide-field imaging of the propagation of electrical stimuli in gap-junction-coupled cell networks. Membrane electromotility-induced cell deformation may be useful as a reporter of electrical activity. © 2012 Biophysical Society.
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