TTF-1 Action on the Transcriptional Regulation of Cyclooxygenase-2 Gene in the Rat Brain
- Authors
- Yun, Chang Ho; Kim, Jae Geun; Park, Byong Seo; Lee, Hye Myeong; Kim, Dong Hee; Kim, Eun Ok; Park, Joong Jean; Park, Jeong Woo; Damante, Giuseppe; Kim, Young Il; Lee, Byung Ju
- Issue Date
- 13-12월-2011
- Publisher
- PUBLIC LIBRARY SCIENCE
- Keywords
- TTF-1 Action on Cyclooxygenase-2 Gene
- Citation
- PLOS ONE, v.6, no.12
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLOS ONE
- Volume
- 6
- Number
- 12
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/110912
- DOI
- 10.1371/journal.pone.0028959
- ISSN
- 1932-6203
- Abstract
- We have recently found that thyroid transcription factor-1 (TTF-1), a homeodomain-containing transcription factor, is postnatally expressed in discrete areas of the hypothalamus and closely involved in neuroendocrine functions. We now report that transcription of cyclooxygenase-2 (COX-2), the rate limiting enzyme in prostaglandin biosynthesis, was inhibited by TTF-1. Double immunohistochemistry demonstrated that TTF-1 was expressed in the astrocytes and endothelial cells of blood vessel in the hypothalamus. Promoter assays and electrophoretic mobility shift assays showed that TTF-1 inhibited COX-2 transcription by binding to specific binding domains in the COX-2 promoter. Furthermore, blocking TTF-1 synthesis by intracerebroventricular injection of an antisense oligomer induced an increase of COX-2 synthesis in non-neuronal cells of the rat hypothalamus, and resulted in animals' hyperthermia. These results suggest that TTF-1 is physiologically involved in the control of thermogenesis by regulating COX-2 transcription in the brain.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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