Pusillimonas harenae sp nov., isolated from a sandy beach, and emended description of the genus Pusilhmonas

Abstract

A Gram-stain-negative, motile bacterium with two lateral flagella, designated strain B201(T), was isolated from beach sand from the Taean coast in South Korea. Cells were ovoid rods and positive for catalase and oxidase. Growth of strain B201(T) was observed between 15 and 45 degrees C (optimum, 30 degrees C) and between pH 5.0 and 9.0 (optimum, pH 6.0-7.5). Strain B201(T) contained ubiquinone Q-8 as the major isoprenoid quinone, but MK-6 was also present as a minor quinone. The major fatty acids of strain B201(T) were C(17:0) cyclo, C(16:0), summed feature 2 (iso-C(16:1) I/C(14:0) 3-OH and/or C(12:0) ALDE), C(12:0) and C(19:0) cyclo omega 8c. The major cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and three aminolipids. The genomic DNA G+C content was 53.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a phyletic lineage with Pusillimonas ginsengisoli DCY25(T) within the genus Pusillimonas. Strain B201(T) was most closely related to P. ginsengisoli DCY25(T) and Pusillimonas soli MJ07(T) with similarities of 98.6 and 97.50/o, respectively. However, DNA DNA relatedness values of strain B201(T) with P. ginsengisoli DCY25(T) and P. soli MJ07(T) were 30.2 +/- 5.4 and 4.9 +/- 1.8 %, respectively. On the basis of chemotaxonomic data and molecular properties, strain B201(T) represents a novel species of the genus Pusillimonas, for which the name Pusillimonas harenae sp. nov. is proposed; the type strain is B201(T) (=KACC 14927(T)=JCM 16917(T)). An emended description of the genus Pusillimonas is given.