Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of the periplasmic domain of outer membrane protein A from Acinetobacter baumannii
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jeong Soon | - |
dc.contributor.author | Lee, Woo Cheol | - |
dc.contributor.author | Choi, Saehae | - |
dc.contributor.author | Yeo, Kwon Joo | - |
dc.contributor.author | Song, Jung Hyun | - |
dc.contributor.author | Han, Young-Hyun | - |
dc.contributor.author | Lee, Je Chul | - |
dc.contributor.author | Kim, Seung Il | - |
dc.contributor.author | Jeon, Young Ho | - |
dc.contributor.author | Cheong, Chaejoon | - |
dc.contributor.author | Kim, Hye-Yeon | - |
dc.date.accessioned | 2021-09-07T05:38:11Z | - |
dc.date.available | 2021-09-07T05:38:11Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.issn | 2053-230X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111032 | - |
dc.description.abstract | Outer membrane protein A from Acinetobacter baumannii (AbOmpA) is a major outer membrane protein and a key player in the bacterial pathogenesis that induces host cell death. AbOmpA is presumed to consist of an N-terminal beta-barrel transmembrane domain and a C-terminal periplasmic OmpA-like domain. In this study, the recombinant C-terminal periplasmic domain of AbOmpA was overexpressed in Escherichia coli, purified and crystallized using the vapour-diffusion method. A native diffraction data set was collected to a resolution of 2.0 angstrom using synchrotron radiation. The space group of the crystal was P21, with unit-cell parameters a = 58.24, b = 98.59, c = 97.96 angstrom, beta = 105.92 degrees. The native crystal contained seven or eight molecules per asymmetric unit and had a calculated Matthews coefficient of 2.93 or 2.56 angstrom 3 Da-1. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | INT UNION CRYSTALLOGRAPHY | - |
dc.subject | PEPTIDOGLYCAN RECOGNITION | - |
dc.subject | EPITHELIAL-CELLS | - |
dc.subject | MOTB | - |
dc.title | Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of the periplasmic domain of outer membrane protein A from Acinetobacter baumannii | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jeon, Young Ho | - |
dc.identifier.doi | 10.1107/S1744309111038401 | - |
dc.identifier.scopusid | 2-s2.0-83055169789 | - |
dc.identifier.wosid | 000297741100013 | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.67, pp.1531 - 1533 | - |
dc.relation.isPartOf | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.volume | 67 | - |
dc.citation.startPage | 1531 | - |
dc.citation.endPage | 1533 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Crystallography | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.subject.keywordPlus | PEPTIDOGLYCAN RECOGNITION | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | MOTB | - |
dc.subject.keywordAuthor | OmpA | - |
dc.subject.keywordAuthor | Acinetobacter baumannii | - |
dc.subject.keywordAuthor | peptidoglycan | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.