Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Survival and colonization of Escherichia coli O157:H7 on spinach leaves as affected by inoculum level and carrier, temperature and relative humidity

Authors
Choi, S.Bang, J.Kim, H.Beuchat, L. R.Ryu, J. -H
Issue Date
12월-2011
Publisher
WILEY-BLACKWELL
Keywords
biofilm; Escherichia coli O157:H7; mesophilic aerobic bacteria; moulds and yeasts; spinach
Citation
JOURNAL OF APPLIED MICROBIOLOGY, v.111, no.6, pp.1465 - 1472
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF APPLIED MICROBIOLOGY
Volume
111
Number
6
Start Page
1465
End Page
1472
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/111040
DOI
10.1111/j.1365-2672.2011.05175.x
ISSN
1364-5072
Abstract
Aims: To determine survival and colonization of Escherichia coli O157:H7 on spinach leaves as affected by inoculum level and carrier, temperature and relative humidity (r.h.). Methods and Results: Spinach leaves were inoculated with suspensions of E. coli O157:H7 in distilled water (DW) and 0.1% peptone water (PW) and incubated at 4, 12 and 25 degrees C and 43, 85 and 100% r.h. The number of E. coli O157:H7 on leaves (5.6 or 1 9 log CFU per leaf) inoculated using DW as a carrier medium increased significantly at 25 degrees C and 100% r.h. within 120 h but remained constant or decreased significantly under other test conditions. E. coli O157:H7 on leaves (5.4 log CFU per leaf) inoculated using PW as a carrier increased significantly within 72 and 24 h, respectively, at 12 or 25 degrees C and 100% r.h.; counts using a low inoculum (2.2 log CFU per leaf) increased significantly within 24 h at 25 degrees C. Conclusions: Escherichia coli O157: H7 can colonize on spinach leaves at 12 or 25 degrees C in a 100% r.h. environment. Organic matter in the inoculum carrier may provide protection and nutrients which enhance survival and colonization. Significance and Impact of the Study: Colonization of E. coli O157:H7 on spinach leaves as affected by organic matter in the inoculum, temperature and r.h. was determined. These observations will be useful when developing strategies to prevent growth of E. coli O157:H7 on pre- and postharvest spinach.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Ryu, Jee Hoon photo

Ryu, Jee Hoon
융합생명공학과
Read more

Altmetrics

Total Views & Downloads

BROWSE