Leaf proteome analysis of wheat-rye translocation lines

Abstract

Wheat-rye translocation lines were developed to produce a main crop resistant to biological and physical stress. Parental line of rye cv. "Chaupon" contains 2RL chromatin to harbor resistance genes for powdery mildew and leaf rust. In order to 2RL-derived rye proteins and 2RL-perbuted proteins in wheat-rye translocation lines, the gel-based proteomics employed with "Coker 797" (non-2RL) and near isogenic line (stabilized 2RL). The leaf proteome was resolved on 2D-gel, and 216 spots were selected for mass spectrometry analysis by image analyzer. A total of 90 proteins with isoform (65 non-reductant proteins) were identified with the identification success rate of 42% using MALDI MS or MS/MS. The identified proteins were classified by functional annotation based on biological processes: carbohydrate metabolic process (11%), cellular amino acid metabolic process (6%), DNA integration/replication (3%), lipid metabolic process (3%), photosynthesis (23%), protein modification/biosynthesis (11%), response to stimulus (8%), transcription and translation (6%), transport (9%), unknown (12%), other biological processes (8%). A total of 24 proteins were differentially expressed between non-2RL and 2RL lines, in which beta-glucosidase, in particular, originated from the chromosome 2RL of rye, was exclusively appeared in NIL. In addition, small Ras-related GTP binding-protein assigned to wheat was predominantly found in 2RL rye chromatin-possessing NIL. These results suggested that the acquired genetic traits obtained from rye 2RL enhance the resistance to biotic and abiotic stress such as Dehydration-induced protein; heat shock protein (HSP 26.6 and 26.6B) in wheat-rye translocation lines by altered the proteome expression.