Human Feeder Cells Can Support the Undifferentiated Growth of Human and Mouse Embryonic Stem Cells Using Their Own Basic Fibroblast Growth Factors
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Yong | - |
dc.contributor.author | Kim, Ji Hea | - |
dc.contributor.author | Lee, Seung Jin | - |
dc.contributor.author | Choi, In Young | - |
dc.contributor.author | Park, Seh Jong | - |
dc.contributor.author | Lee, Se Ryeon | - |
dc.contributor.author | Sung, Hwa Jung | - |
dc.contributor.author | Yoo, Young Do | - |
dc.contributor.author | Geum, Dong Ho | - |
dc.contributor.author | Choi, Chul Won | - |
dc.contributor.author | Kim, Sun Haeng | - |
dc.contributor.author | Kim, Byung Soo | - |
dc.date.accessioned | 2021-09-07T06:35:43Z | - |
dc.date.available | 2021-09-07T06:35:43Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-11 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111231 | - |
dc.description.abstract | In the culture system using human feeder cells, the mechanism through which these cells support undifferentiated growth of embryonic stem cells (ESCs) has not been well investigated. Here, we explored the mechanisms of 3 kinds of human feeder cells, including human placental cells from the chorionic plate, human bone marrow stromal cells, and human foreskin fibroblasts. First, we determined that undifferentiated growth of 2 kinds each of human (H1 and HSF6) and mouse (D3 and CE3) ESCs was possible in all human feeder cell types tested (human placental cells, human bone marrow stromal cells, and human foreskin fibroblasts), without the need for exogenous cytokine supplementation including basic fibroblast growth factor (bFGF) and leukemia inhibitory factor. We then prepared their corresponding endogenous bFGF-knockout feeders using siRNA and tried to maintain human and mouse ESCs in their undifferentiated state; however, neither human nor mouse ESCs could be maintained in bFGF-knockout human feeder cells. The expressions of stemness markers such as Oct-4 and Nanog were significantly decreased in the bFGF-knockout group compared with those in the controls, and differentiation had already occurred, despite the undifferentiated morphologic appearance of the ESCs. In conclusion, human feeder cells are able to support the undifferentiated growth of human and mouse ESCs via bFGF synthesis. Further, a bFGF-dependent pathway might be crucial for maintaining the undifferentiated characteristics of mouse and human ESCs. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MARY ANN LIEBERT INC | - |
dc.subject | HUMAN PLACENTA | - |
dc.subject | CULTURE | - |
dc.subject | LINES | - |
dc.subject | PROPAGATION | - |
dc.subject | DERIVATION | - |
dc.subject | FGF | - |
dc.subject | LIF | - |
dc.title | Human Feeder Cells Can Support the Undifferentiated Growth of Human and Mouse Embryonic Stem Cells Using Their Own Basic Fibroblast Growth Factors | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Yong | - |
dc.contributor.affiliatedAuthor | Sung, Hwa Jung | - |
dc.contributor.affiliatedAuthor | Yoo, Young Do | - |
dc.contributor.affiliatedAuthor | Geum, Dong Ho | - |
dc.contributor.affiliatedAuthor | Choi, Chul Won | - |
dc.contributor.affiliatedAuthor | Kim, Sun Haeng | - |
dc.contributor.affiliatedAuthor | Kim, Byung Soo | - |
dc.identifier.doi | 10.1089/scd.2010.0496 | - |
dc.identifier.scopusid | 2-s2.0-84862833041 | - |
dc.identifier.wosid | 000296587400007 | - |
dc.identifier.bibliographicCitation | STEM CELLS AND DEVELOPMENT, v.20, no.11, pp.1901 - 1910 | - |
dc.relation.isPartOf | STEM CELLS AND DEVELOPMENT | - |
dc.citation.title | STEM CELLS AND DEVELOPMENT | - |
dc.citation.volume | 20 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1901 | - |
dc.citation.endPage | 1910 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.subject.keywordPlus | HUMAN PLACENTA | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | LINES | - |
dc.subject.keywordPlus | PROPAGATION | - |
dc.subject.keywordPlus | DERIVATION | - |
dc.subject.keywordPlus | FGF | - |
dc.subject.keywordPlus | LIF | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.